Chantler S, Devries E, Allen P R, Hurn B A
J Immunol Methods. 1976;13(3-4):367-80. doi: 10.1016/0022-1759(76)90083-1.
Sera from adults with toxoplasmosis and individuals with anti nuclear antibodies were examined for the presenceof specific IgM and IgG antibodies and for the occurrence of anti-immunoglobulin ("RF") activity both before and after absorption with freeze-dried preparations of protein A-containing Staphylococcus aureus. Such absorption removed most of the IgG present in sera without interfering with the detection of IgM antibody by immunofluorescence, but was relatively ineffective in the removal of "RF". "RF" activity, demonstrable by slide agglutination, was removed by absorption with latex coated with IgG. In order to prevent the possibility of false positive IgM staining in immunofluorescence tests, it is concluded that sequential absorption with latex-IgG particles and protein A-positive staphylococci is essential. Such absorption is technically simple and can be performed on small volumes of serum.
对患有弓形虫病的成年人以及具有抗核抗体的个体的血清进行检测，以确定在用含蛋白A的金黄色葡萄球菌冻干制剂吸收前后，特异性IgM和IgG抗体的存在情况以及抗免疫球蛋白（“RF”）活性的发生情况。这种吸收去除了血清中存在的大部分IgG，而不干扰通过免疫荧光检测IgM抗体，但在去除“RF”方面相对无效。通过玻片凝集可证明的“RF”活性，在用包被有IgG的乳胶吸收后被去除。为了防止免疫荧光试验中出现假阳性IgM染色的可能性，得出结论认为，用乳胶-IgG颗粒和蛋白A阳性葡萄球菌进行顺序吸收是必不可少的。这种吸收在技术上很简单，可以在少量血清上进行。
