Federspiel M J, Bates P, Young J A, Varmus H E, Hughes S H
Advanced BioScience Laboratories-Basic Research Program, National Cancer Institute-Frederick Cancer Research and Development Center, MD 21702-1201.
Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):11241-5. doi: 10.1073/pnas.91.23.11241.
Avian leukosis viruses (ALVs) have been used extensively as genetic vectors in avian systems, but their utility in mammals or mammalian cell lines is compromised by inefficient viral entry. We have overcome this limitation by generating transgenic mice that express the receptor for the subgroup A ALV under the control of the chicken alpha sk-actin promoter. The skeletal muscles of these transgenic animals are susceptible to efficient infection by subgroup A ALV. Because infection is restricted to cell lineages that express the transgene, the method has utility for studies of development and oncogenesis and will provide models for tissue-specific gene therapy.
禽白血病病毒（ALVs）已在禽类系统中广泛用作基因载体，但它们在哺乳动物或哺乳动物细胞系中的效用因病毒进入效率低下而受到影响。我们通过生成在鸡α-肌动蛋白启动子控制下表达A亚群ALV受体的转基因小鼠克服了这一限制。这些转基因动物的骨骼肌易受A亚群ALV的有效感染。由于感染仅限于表达转基因的细胞谱系，该方法可用于发育和肿瘤发生的研究，并将为组织特异性基因治疗提供模型。
