人体中针对主要免疫原扁卷螺血蓝蛋白（HPH）的特异性类别抗体滴度（酶联免疫吸附测定法）
Class-specific antibody titres (ELISA) against the primary immunogen Helix pomatia haemocyanin (HPH) in man.
作者信息
Weits J, de Gast G C, The T H, Esselink M T, Deelder A M, Petrovic M, Mandema E
出版信息
Clin Exp Immunol. 1978 Jun;32(3):443-50.
Using the indirect ELISA technique, IgM, IgG and IgA antibody titres against the primary test immunogen Helix pomatia haemocyanin (HPH) could be measured. All twenty-seven normal volunteers (age range 26-74 years) developed maximal or high IgM, IgG and IgA antibody titres 2 weeks after primary immunization with 1.0 mg HPH subcutaneously. Lower dose immunization resulted in submaximal responses. Titre kinetics in the three Ig classes correlated in height and time per person. There was no significant difference in antibody response between two adult age groups. Secondary immunization after 6 weeks, again with 1.0 mg HPH, when high titres were still present, resulted in a small titre increase in all three Ig classes. Comparison with the tanned red cell agglutination technique used previously, in combination with the 2-mercaptoethanol treatment of sera, showed the unreliability of 7S titres measured thus early in the primary immune response. Investigation of four patients with humoral immunodeficiency confirmed the class specificity of the test.
采用间接酶联免疫吸附测定技术，可检测针对主要试验免疫原——蜗牛血蓝蛋白（HPH）的IgM、IgG和IgA抗体滴度。所有27名正常志愿者（年龄范围26 - 74岁）在皮下注射1.0 mg HPH进行初次免疫后2周，IgM、IgG和IgA抗体滴度达到最高或较高水平。较低剂量免疫导致反应未达最大值。每个人的三种免疫球蛋白类别中的滴度动力学在高度和时间上具有相关性。两个成年年龄组之间的抗体反应无显著差异。6周后再次用1.0 mg HPH进行二次免疫，此时仍存在高滴度，结果在所有三种免疫球蛋白类别中滴度均有小幅升高。与先前使用的鞣酸红细胞凝集技术以及血清的2 - 巯基乙醇处理相结合进行比较，结果表明在初次免疫反应早期如此测定的7S滴度不可靠。对4名体液免疫缺陷患者的研究证实了该检测的类别特异性。
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