哥斯达黎加消化不良患者群体中的幽门螺杆菌。
Helicobacter pylori in a Costa Rican dyspeptic patient population.
作者信息
Morera-Brenes B, Sierra R, Barrantes R, Jonasson J, Nord C E
机构信息
Instituto de Investigaciones en Salud, Universidad de Costa Rica, San José.
出版信息
Eur J Clin Microbiol Infect Dis. 1994 Mar;13(3):253-7. doi: 10.1007/BF01974546.
PMID:8050440
Abstract
Gastric biopsies from 65 Costa Rican dyspeptic patients were investigated for the presence of Helicobacter pylori DNA by polymerase chain reaction (PCR) amplification of 16S rRNA sequences. Both frozen and paraffin-embedded samples were used, and the results were compared with bacterial culture and histological examination. Helicobacter pylori DNA was detected by PCR in 60 (92%) of the patients, and Helicobacter pylori strains confirmed by PCR could be isolated from 37 of them. Altogether, 59 patients were shown to be infected using the combination of culture and histology as the reference method. The sensitivity of PCR analysis of frozen material was 98% (58/59). The PCR analysis of paraffin-embedded samples seemed less reliable than that of frozen biopsy material.
摘要
通过聚合酶链反应（PCR）扩增16S rRNA序列，对65名患有消化不良的哥斯达黎加患者的胃活检样本进行了幽门螺杆菌DNA检测。研究使用了冷冻样本和石蜡包埋样本，并将结果与细菌培养和组织学检查进行了比较。通过PCR在60名（92%）患者中检测到幽门螺杆菌DNA，其中37名患者经PCR确认的幽门螺杆菌菌株能够被分离出来。以培养和组织学相结合作为参考方法，总共显示59名患者受到感染。冷冻材料的PCR分析灵敏度为98%（58/59）。石蜡包埋样本的PCR分析似乎不如冷冻活检材料可靠。
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