Gleaves C A, Lee C F, Bustamante C I, Meyers J D
Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, Seattle, Washington.
J Clin Microbiol. 1988 Sep;26(9):1623-5. doi: 10.1128/jcm.26.9.1623-1625.1988.
The laboratory diagnosis of varicella-zoster virus (VZV) infection was reevaluated by direct immunofluorescent-antibody staining (DFA) and centrifugation culture with newly available murine monoclonal antibodies. Specimen smears were examined by DFA using monoclonal antibodies to VZV and to herpes simplex virus types 1 and 2. Specimens were also inoculated into shell vials for centrifugation culture and into standard tube cell culture. Of 68 specimens tested from 60 patients, 39 (57%) were positive for VZV by at least one method. DFA was positive in 36 of 39 (92%); centrifugation culture was positive at 24 h in 23 of 39 (59%) and at 48 h in 31 of 39 (79%); and standard culture was positive in 25 of 39 (64%). Twenty-three of the 39 positive specimens (59%) were positive by all three techniques. Forty-three of the 60 patients were considered to have VZV by clinical criteria, and 35 of these 43 (81%) had laboratory confirmation of the diagnosis. These data confirm that DFA is the method of choice for the rapid laboratory confirmation of VZV infection. The centrifugation culture assay can provide an alternative method to DFA for the laboratory diagnosis of VZV infection.
采用新获得的鼠单克隆抗体，通过直接免疫荧光抗体染色（DFA）和离心培养对水痘-带状疱疹病毒（VZV）感染的实验室诊断进行了重新评估。使用针对VZV以及1型和2型单纯疱疹病毒的单克隆抗体，通过DFA对标本涂片进行检查。标本还接种到用于离心培养的壳瓶和标准试管细胞培养中。在对60例患者检测的68份标本中，至少有一种方法检测出39份（57%）VZV呈阳性。39份中有36份（92%）DFA呈阳性；离心培养在24小时时39份中有23份（59%）呈阳性，48小时时39份中有31份（79%）呈阳性；标准培养39份中有25份（64%）呈阳性。39份阳性标本中有23份（59%）三种技术均呈阳性。60例患者中有43例根据临床标准被认为感染VZV，这43例中的35例（81%）经实验室确诊。这些数据证实，DFA是快速实验室确诊VZV感染的首选方法。离心培养测定可为VZV感染的实验室诊断提供一种替代DFA的方法。
