E试验与琼脂稀释法用于淋病奈瑟菌抗菌药敏试验的比较。
Comparison of E test with agar dilution for antimicrobial susceptibility testing of Neisseria gonorrhoeae.
作者信息
Van Dyck E, Smet H, Piot P
机构信息
Institute of Tropical Medicine, Division of Microbiology, Antwerp, Belgium.
出版信息
J Clin Microbiol. 1994 Jun;32(6):1586-8. doi: 10.1128/jcm.32.6.1586-1588.1994.
Abstract
A collection of 150 Neisseria gonorrhoeae isolates from Africa, where various resistance mechanisms among N. gonorrhoeae isolates are common, was used to the compare E test (AB Biodisk, Solna, Sweden) with agar dilution susceptibility testing. MICs obtained by the E test agreed within 1 log2 concentration by the agar dilution method for 97.5, 97.3, 96.6, 94, and 84.7% of the tested isolates for penicillin, ciprofloxacin, chloramphenicol, tetracycline, and trimethoprim-sulfamethoxazole, respectively. No significant difference in susceptibility categorization was observed between either method. The E test is an attractive alternative to the agar dilution technique and is a more appropriate method for N. gonorrhoeae susceptibility testing in developing countries.
摘要
收集了150株来自非洲的淋病奈瑟菌分离株，在非洲淋病奈瑟菌分离株中各种耐药机制很常见，用其将E试验（AB Biodisk，瑞典索尔纳）与琼脂稀释药敏试验进行比较。对于青霉素、环丙沙星、氯霉素、四环素和甲氧苄啶-磺胺甲恶唑，通过E试验获得的最低抑菌浓度（MIC）与琼脂稀释法在1个对数2浓度范围内相符的受试分离株比例分别为97.5%、97.3%、96.6%、94%和84.7%。两种方法在药敏分类上均未观察到显著差异。E试验是琼脂稀释技术的一种有吸引力的替代方法，是发展中国家进行淋病奈瑟菌药敏试验更合适的方法。
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