Schaeffer W I, Olson L D, Barile M F, Sun F W
Department of Microbiology and Molecular Genetics, Colleges of Agriculture and Life Sciences, University of Vermont, Burlington 05405.
J Bacteriol. 1991 Feb;173(4):1382-7. doi: 10.1128/jb.173.4.1382-1387.1991.
Mycoplasma hyorhinis coisolates with the mitochondria of the cells in which it is carried as an infection. Since both mitochondria and mycoplasmas synthesize DNA by using the prokaryotic DNA polymerase gamma, the use of aphidicolin, which inhibits eukaryotic DNA polymerase alpha, allows for selective synthesis of mycoplasmal and mitochondrial DNA. The restriction patterns of mitochondria and mycoplasmas can easily be differentiated from each other in mixtures of both DNAs. Thus, it is possible to study the molecular biology of this noncultivable mycoplasma in situ rather than after growth in artificial media, with its potential genetic consequences during adjustment to axenic growth.
猪鼻支原体与它作为感染携带的细胞中的线粒体共同分离。由于线粒体和支原体都通过使用原核DNA聚合酶γ来合成DNA，使用抑制真核DNA聚合酶α的阿非迪霉素，可实现支原体和线粒体DNA的选择性合成。在两种DNA的混合物中，线粒体和支原体的限制性图谱能够很容易地相互区分。因此，有可能原位研究这种不可培养支原体的分子生物学，而不是在人工培养基中生长后进行研究，同时也能研究其在适应无菌生长过程中的潜在遗传后果。
