Connolly Keith, Culver Gloria
Departments of Biology and of Biochemistry and Biophysics, University of Rochester, Rochester, NY 14627, USA.
Trends Biochem Sci. 2009 May;34(5):256-63. doi: 10.1016/j.tibs.2009.01.011. Epub 2009 Apr 17.
The ribosome is an essential ribonucleoprotein enzyme, and its biogenesis is a fundamental process in all living cells. Recent X-ray crystal structures of the bacterial ribosome and new technologies have allowed a greater interrogation of in vitro ribosome assembly; however, substantially less is known about ribosome biogenesis in vivo. Ongoing investigations are focused on elucidating the cellular processes that facilitate biogenesis of the ribosomal subunits, and many extraribosomal factors, including modification enzymes, remodeling enzymes and GTPases, are being uncovered. Moreover, specific roles for ribosome biogenesis factors in subunit maturation are now being elaborated. Ultimately, such studies will reveal a more complete understanding of processes at work in in vivo ribosome biogenesis.
核糖体是一种必需的核糖核蛋白酶，其生物合成是所有活细胞中的一个基本过程。最近细菌核糖体的X射线晶体结构和新技术使得对体外核糖体组装有了更深入的研究；然而，对于体内核糖体生物合成的了解却少得多。正在进行的研究集中于阐明促进核糖体亚基生物合成的细胞过程，并且许多核糖体外部因子，包括修饰酶、重塑酶和GTP酶，正在被发现。此外，现在正在详细阐述核糖体生物合成因子在亚基成熟中的具体作用。最终，此类研究将揭示对体内核糖体生物合成过程更全面的理解。
