Tyan M L
Veterans Affairs Medical Center, West Los Angeles, California 90073.
Proc Soc Exp Biol Med. 1994 Jan;205(1):85-8. doi: 10.3181/00379727-205-43682.
Pregnant mice which in theory differ only in the region of the major histocompatibility complex (MHC) on chromosome 17 (C57BL/10, the inbred partner [host strain], and B10.D2, B10.BR, B10.A, B10.A[2R], B10.A[5R], B10.A[15R] and B10.A[18R]) were sacrificed on the 11th and 18th days of gestation, and the fetuses were sexed and weighed. Fetuses from reciprocal crosses between B10.A and B10.BR, B10.D2 and C57BL/10 were weighed and sexed on the 18th day of gestation. It was found that (i) fetal weights were not significantly different among the strains examined on day 11 (B10.BR, B10.A[15R] and B10.A[18R]), (ii) B10.BR fetuses of both sexes weighed significantly less than fetuses from the other strains on day 18, (iii) B10.D2 18-day-old male but not female fetuses were heavier than the males from the other strains (this difference was not present when corrections for litter size were made), (iv) the fetuses from the B10.A x B10.BR cross were the smallest, those from the B10.D2 x B10.A cross the largest, and those from the B10.A x C57BL/10 crosses intermediate, and (v) maternal effects were noted in the B10.A x B10.BR and B10.A x B10.D2 but not the B10.A x C57BL/10 crosses. The results suggest that there are two or more MHC associated loci that influence growth rate in late gestation. Among the candidate genes are Ped and Igfr II.
理论上仅在17号染色体上的主要组织相容性复合体（MHC）区域存在差异的怀孕小鼠（C57BL/10，近交系伙伴[宿主品系]，以及B10.D2、B10.BR、B10.A、B10.A[2R]、B10.A[5R]、B10.A[15R]和B10.A[18R]）在妊娠第11天和第18天被处死，对胎儿进行性别鉴定和称重。对B10.A与B10.BR、B10.D2与C57BL/10之间的正反交胎儿在妊娠第18天进行称重和性别鉴定。结果发现：（i）在第11天检测的品系（B10.BR、B10.A[15R]和B10.A[18R]）中胎儿体重无显著差异；（ii）第18天，B10.BR两性胎儿的体重均显著低于其他品系的胎儿；（iii）B10.D2妊娠18天的雄性胎儿而非雌性胎儿比其他品系的雄性胎儿重（进行窝仔数校正后这种差异不存在）；（iv）B10.A×B10.BR杂交的胎儿最小，B10.D2×B10.A杂交的胎儿最大，B10.A×C57BL/10杂交的胎儿居中；（v）在B10.A×B10.BR和B10.A×B10.D2杂交中观察到母体效应，而在B10.A×C57BL/10杂交中未观察到。结果表明，存在两个或更多与MHC相关的位点影响妊娠后期的生长速率。候选基因中有Ped和Igfr II。
