Goldstein E, Bartlema H C, van der Ploeg M, van Duijn P, van der Stap J G, Lippert W
J Infect Dis. 1978 Sep;138(3):299-311. doi: 10.1093/infdis/138.3.299.
The role of lysosomal enzymes in the inactivation of inhaled bacteria by alveolar macrophages was studied in rats infected with aerosols of Staphylococcus aureus and then exposed for 5 hr to 2.5 ppm of ozone to determine whether pollutant-induced defects in phagocytic killing were associated with reduction in enzyme activity. Rates of bacterial ingestion and the activities of cellular acid phosphatase and beta-glucuronidase were measured simultaneously in in situ perfused right lungs by sequential staining of frozen sections for enzyme and bacteria. Quantitative measurements of enzyme activity within macrophages without ingested bacteria were made with a computer-controlled cytospectrophotometry system. Exposure to ozone resulted in diminished rates of bacterial clearance and ingestion, large increases in numbers of intra- and extracellular staphylococcal microcolonies, and an absence of enzyme activity for macrophages containing bacterial microcolonies. Enzyme activity was unimpaired in macrophages without ingested bacteria. These results, in which absence of enzyme activity occurred only in macrophages subjected to the dual insults of ozone exposure and ingested bacteria, prove a relationship between impairment in bactericidal capacity and cellular activities of lysosomal enzymes.
在感染金黄色葡萄球菌气溶胶的大鼠中，研究了溶酶体酶在肺泡巨噬细胞对吸入细菌的失活作用中的角色。这些大鼠随后暴露于2.5 ppm的臭氧中5小时，以确定污染物诱导的吞噬杀伤缺陷是否与酶活性降低有关。通过对冷冻切片进行酶和细菌的顺序染色，同时测量原位灌注右肺中细菌摄取率以及细胞酸性磷酸酶和β-葡萄糖醛酸酶的活性。使用计算机控制的细胞分光光度测定系统对未摄取细菌的巨噬细胞内的酶活性进行定量测量。暴露于臭氧导致细菌清除和摄取率降低，细胞内和细胞外葡萄球菌微菌落数量大幅增加，并且含有细菌微菌落的巨噬细胞缺乏酶活性。未摄取细菌的巨噬细胞中的酶活性未受损。这些结果表明，仅在遭受臭氧暴露和摄取细菌双重损伤的巨噬细胞中才出现酶活性缺失，这证明了杀菌能力受损与溶酶体酶的细胞活性之间存在关联。
