Mulder A H, Broekroelofs J, Horst G, Limburg P C, Nelis G F, Kallenberg C G
Department of Clinical Immunology, University Hospital Groningen, The Netherlands.
Clin Exp Immunol. 1994 Mar;95(3):490-7. doi: 10.1111/j.1365-2249.1994.tb07024.x.
ANCA were detected by indirect immunofluorescence in 34 out of 67 patients with ulcerative colitis (UC, 51%) and in 14 out of 35 patients with Crohn's disease (CD, 40%). All but one ANCA-positive sera produced a perinuclear pattern of fluorescence (P-ANCA) on ethanol-fixed neutrophils. On paraformaldehyde-fixed neutrophils 76% of P-ANCA-positive sera in UC and 50% of P-ANCA-positive sera in CD produced cytoplasmic fluorescence, indicating that, indeed, cytoplasmic antigens are recognized by a considerable number of these sera. By Western blot analysis using whole neutrophil extract as a substrate 46% of sera from patients with UC and 32% of sera from patients with CD showed reactivity with either lactoferrin, polypeptides occurring as a doublet of 66/67 kD mol. wt, or polypeptides occurring as a doublet of 63/54 kD mol. wt, respectively. Identical patterns of reactivity have been observed among P-ANCA-positive sera from patients with autoimmune liver disease and rheumatoid arthritis. These data suggest that ANCA of restricted specificities are not specific for inflammatory bowel disease (IBD), but are present in diverse conditions characterized by chronic idiopathic inflammation.
在67例溃疡性结肠炎（UC）患者中，34例（51%）通过间接免疫荧光法检测到抗中性粒细胞胞浆抗体（ANCA）；在35例克罗恩病（CD）患者中，14例（40%）检测到ANCA。除1份ANCA阳性血清外，所有ANCA阳性血清在乙醇固定的中性粒细胞上均产生核周荧光模式（P-ANCA）。在多聚甲醛固定的中性粒细胞上，UC中76%的P-ANCA阳性血清和CD中50%的P-ANCA阳性血清产生胞浆荧光，这表明相当一部分此类血清确实识别胞浆抗原。通过以全中性粒细胞提取物为底物的蛋白质印迹分析，UC患者血清中46%以及CD患者血清中32%分别与乳铁蛋白、分子量为66/67 kD的双峰多肽或分子量为63/54 kD的双峰多肽发生反应。在自身免疫性肝病和类风湿关节炎患者的P-ANCA阳性血清中观察到相同的反应模式。这些数据表明，具有受限特异性的ANCA并非炎症性肠病（IBD）所特有，而是存在于以慢性特发性炎症为特征的多种病症中。
