Riddle D L, Giger O, Miller L, Hall G S, Woods G L
Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia.
Am J Clin Pathol. 1994 Apr;101(4):438-42. doi: 10.1093/ajcp/101.4.438.
To determine the reliability of the Baxter MicroScan Yeast Identification Panel, processed by the Walkaway-96, and the Vitek Yeast Biochemical Card, 150 clinical yeast isolates (30 Candida albicans, 67 Candida species, not albicans, 26 Torulopsis glabrata, 13 Cryptococcus neoformans, 4 Saccharomyces cerevisiae, 6 Trichosporon beigelii, 3 Rhodotorula species, and 1 Geotrichum species) were tested on both systems. Results were compared with those obtained by the API 20C and the appearance of yeast cells on cornmeal Tween-80 agar. After inoculation of each system, results were available in 4 hours with MicroScan panels, 24-48 hours with Vitek cards, and 72 hours with the API 20C strips. On initial testing, 101 (67%) and 128 (85%) isolates, respectively, were correctly identified by MicroScan and Vitek. After repeat testing, the number of correctly identified isolates increased to 123 (82%) by MicroScan and to 142 (95%) by Vitek. Yeasts most commonly misidentified were Candida tropicalis, T glabrata, and Candida parapsilosis by MicroScan and C tropicalis and T glabrata by Vitek.
为了确定由Walkaway - 96处理的百特微生物自动鉴定仪酵母鉴定板以及Vitek酵母生化鉴定卡的可靠性，我们使用这两种系统对150株临床酵母分离株（30株白色念珠菌、67株非白色念珠菌属念珠菌、26株光滑假丝酵母菌、13株新生隐球菌、4株酿酒酵母、6株白吉利丝孢酵母、3株红酵母属菌株以及1株地丝菌属菌株）进行了检测。将结果与通过API 20C以及玉米粉吐温80琼脂上酵母细胞的形态所获得的结果进行比较。在接种每种系统后，使用微生物自动鉴定仪鉴定板4小时可获得结果，使用Vitek鉴定卡24 - 48小时可获得结果，使用API 20C鉴定条72小时可获得结果。在初次检测时，微生物自动鉴定仪和Vitek分别正确鉴定了101株（67%）和128株（85%）分离株。经过重复检测后，微生物自动鉴定仪正确鉴定的分离株数量增至123株（82%），Vitek增至142株（95%）。微生物自动鉴定仪最常误鉴定的酵母是热带念珠菌、光滑假丝酵母菌和近平滑念珠菌，Vitek最常误鉴定的是热带念珠菌和光滑假丝酵母菌。
