el-Zaatari M, Pasarell L, McGinnis M R, Buckner J, Land G A, Salkin I F
Department of Pathology, University of Texas Medical Branch, Galveston 77550.
J Clin Microbiol. 1990 Sep;28(9):1938-41. doi: 10.1128/jcm.28.9.1938-1941.1990.
Using 398 isolates of yeasts and yeastlike fungi comprising 9 genera and 26 species, as well as the hyphomycete Geotrichum candidum and the achlorophyllous alga Prototheca wickerhamii, we compared the API 20C yeast identification system with the modified Vitek yeast identification system with an expanded data base. We found 11 discrepancies between the two systems: five (1.3%) of the isolates (Blastoschizomyces capitatus, 1; Candida albicans, 1; Hansenula anomala, 1; Rhodotorula minuta, 2) had biocodes not included in the expanded Vitek data base, and six (1.5%) of the isolates (Candida lusitaniae, 1; Candida parapsilosis, 1; Cryptococcus uniguttulatus, 1; H. anomala, 1; Torulopsis candida, 2) were misidentified by the Vitek system. Overall, the efficacy of the Vitek system compares favorably with that of the API 20C in the identification of clinically important yeasts.
我们使用了398株酵母和类酵母真菌,它们分属于9个属、26个种,还包括丝孢菌白地霉和无叶绿素藻类威克汉姆原藻,将API 20C酵母鉴定系统与具有扩展数据库的改良Vitek酵母鉴定系统进行了比较。我们发现这两种系统之间存在11处差异:有5株(1.3%)分离株(头裂殖酵母1株;白色念珠菌1株;异常汉逊酵母1株;微小红酵母2株)的生物编码未包含在扩展的Vitek数据库中,还有6株(1.5%)分离株(葡萄牙念珠菌1株;近平滑念珠菌1株;单囊隐球菌1株;异常汉逊酵母1株;假丝酵母样球拟酵母2株)被Vitek系统错误鉴定。总体而言,在鉴定临床上重要的酵母方面,Vitek系统的效能与API 20C系统相当。