Doble A, Fox A, Glynn L E, Kingston D
Br J Exp Pathol. 1975 Dec;56(6):537-43.
The intra-articular injection of antigen after immunization with that antigen in FCA, the mycobacterial component of which consisted of live M. avium or dead 125I-M. tuberculosis, resulted in identical chronic disease, experimental allergic arthritis. In the former case live mycobacteria could be isolated from the subcutaneous granuloma which developed at the injection site, and from arthritic joints into which 10 mug wet weight (equivalent of 2 mug dry weight) had been injected with this antigen. However, no mycobacteria could be isolated from other inflamed or normal joints. With 125I-labelled mycobacteria radioactive counting and radioautography clearly showed M. tuberculosis present in the subcutaneous granuloma but not in either injected or uninjected joints. The quantity of mycobacteria which could have been detected if present was estimated to be less than 1 mug dry weight. These results strongly suggest that mycobacteria do not travel from the granuloma depot to the arthritic joint, and that any mechanism one may consider to explain the chronicity of EAA cannot involve the physical presence of mycobacteria in the knee joint.
在用弗氏完全佐剂（FCA）中的该抗原免疫后进行关节腔内注射抗原，其中分枝杆菌成分由活的鸟分枝杆菌或死的125I标记的结核分枝杆菌组成，结果导致相同的慢性疾病，即实验性变应性关节炎。在前一种情况下，可从注射部位形成的皮下肉芽肿以及已注射10微克湿重（相当于2微克干重）该抗原的关节炎关节中分离出活的分枝杆菌。然而，从其他发炎或正常关节中未能分离出分枝杆菌。对于125I标记的分枝杆菌，放射性计数和放射自显影清楚地表明结核分枝杆菌存在于皮下肉芽肿中，但不存在于注射或未注射的关节中。如果存在的话，估计可检测到的分枝杆菌数量少于1微克干重。这些结果强烈表明，分枝杆菌不会从肉芽肿储存部位转移到关节炎关节，并且任何可能用于解释实验性变应性关节炎慢性化的机制都不可能涉及分枝杆菌在膝关节中的实际存在。
