Carr M W, Roth S J, Luther E, Rose S S, Springer T A
Committee on Immunology, Harvard Medical School, Boston, MA.
Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3652-6. doi: 10.1073/pnas.91.9.3652.
We have utilized a transendothelial lymphocyte chemotaxis assay to identify and purify a lymphocyte chemoattractant in supernatants of mitogen-stimulated peripheral blood mononuclear cells. Amino acid sequence analysis revealed identity with monocyte chemoattractant protein 1 (MCP-1), a chemoattractant previously thought to be specific for monocytes. Recombinant MCP-1 is chemoattractive for purified T lymphocytes and for CD3+ lymphocytes in peripheral blood lymphocyte preparations. The T-cell response to MCP-1 is dose-dependent and chemotactic, rather than chemokinetic. Phenotyping of chemoattracted T lymphocytes shows they are an activated memory subset. The response to MCP-1 by T lymphocytes can be duplicated in the absence of an endothelial monolayer and the majority of T-lymphocyte chemotactic activity in mitogen-stimulated peripheral blood mononuclear cell supernatants can be neutralized by antibody to MCP-1. Thus, MCP-1 is the major lymphocyte chemoattractant secreted by mitogen-stimulated peripheral blood mononuclear cells and is capable of acting as a potent T-lymphocyte, as well as monocyte, chemoattractant. This may help explain why monocytes and T lymphocytes of the memory subset are always found together at sites of antigen-induced inflammation.
我们利用跨内皮淋巴细胞趋化试验，从丝裂原刺激的外周血单核细胞的上清液中鉴定并纯化出一种淋巴细胞趋化因子。氨基酸序列分析显示其与单核细胞趋化蛋白1（MCP-1）相同，MCP-1是一种先前被认为对单核细胞具有特异性的趋化因子。重组MCP-1对纯化的T淋巴细胞以及外周血淋巴细胞制剂中的CD3+淋巴细胞具有趋化作用。T细胞对MCP-1的反应呈剂量依赖性且具有趋化性，而非化学促动性。对趋化的T淋巴细胞进行表型分析表明，它们是活化的记忆亚群。在没有内皮单层的情况下，T淋巴细胞对MCP-1的反应仍可重现，并且丝裂原刺激的外周血单核细胞上清液中的大多数T淋巴细胞趋化活性可被抗MCP-1抗体中和。因此，MCP-1是丝裂原刺激的外周血单核细胞分泌的主要淋巴细胞趋化因子，并且能够作为一种有效的T淋巴细胞以及单核细胞趋化因子发挥作用。这可能有助于解释为什么在抗原诱导的炎症部位总是同时发现记忆亚群的单核细胞和T淋巴细胞。
