Krishnasamy S, Makaroff C A
Department of Chemistry, Miami University, Oxford, OH 45056.
Curr Genet. 1993 Jul-Aug;24(1-2):156-63. doi: 10.1007/BF00324680.
The orfB locus of the normal (fertile) and Ogura (male-sterile) radish mitochondrial genomes has been characterized in order to determine if this region, which has previously been correlated with cytoplasmic male sterility (CMS) in Brassica napus cybrids (Bonhomme et al. 1991; Temple et al. 1992), could also be involved in radish CMS. In normal radish, orfB is expressed as a 600-nucleotide (nt) transcript. In Ogura radish, orfB is present as the second gene of a 1200-nt transcript that also contains a 138-codon open reading frame (orf138). Sequences showing similarity to orf138 are present in normal radish, but are not expressed.
为了确定正常(可育)和小仓(雄性不育)萝卜线粒体基因组的orfB基因座是否也与萝卜细胞质雄性不育(CMS)有关,此前该区域已被证明与甘蓝型油菜体细胞杂种的细胞质雄性不育(CMS)相关(Bonhomme等人,1991年;Temple等人,1992年),我们对其进行了特征分析。在正常萝卜中,orfB基因表达为一个600个核苷酸(nt)的转录本。在小仓萝卜中,orfB基因是一个1200 nt转录本的第二个基因,该转录本还包含一个138个密码子的开放阅读框(orf138)。与orf138相似的序列在正常萝卜中也存在,但不表达。