Yamashita T, Sakae K, Ishihara Y, Isomura S, Utagawa E
Department of Virology, Aichi Prefectural Institute of Public Health, Nagoya, Japan.
J Clin Microbiol. 1993 Nov;31(11):2938-43. doi: 10.1128/jcm.31.11.2938-2943.1993.
Cytopathic small round virus (Aichi strain), isolated from a patient with oyster-associated gastroenteritis, showed no reaction in the polymerase chain reaction method for enteroviruses or in the enzyme-linked immunosorbent assay (ELISA) for the five serotypes of astroviruses. Our ELISA was sensitive in detecting the Aichi strain antigen in stool samples, but there was no reaction in this ELISA with any non-Aichi strains of enteric viruses, with such origins as enterovirus, rotavirus, Norwalk virus, calicivirus, or astrovirus. In the ELISA, 13 of 47 stool samples from adult patients in five of nine oyster-associated gastroenteritis outbreaks were positive, but only 1 of 397 pediatric stool samples in Aichi Prefecture was positive. The prevalence rate for Aichi strain antibody was found to be 7.2% for persons aged 7 months to 4 years. The prevalence rate for antibody to Aichi strain increased with age, to about 80% in persons 35 years old. On the basis of the results of the present study, it was hypothesized that Aichi strain could be a new type of small round virus that mainly produces diarrhea in patients in the 15- to 34-year-old age group, 50 to 76% of whom possess neutralizing antibody.
从一名患有与牡蛎相关的肠胃炎患者身上分离出的细胞病变性小圆病毒（爱知株），在针对肠道病毒的聚合酶链反应方法以及针对五种血清型星状病毒的酶联免疫吸附测定（ELISA）中均未出现反应。我们的ELISA在检测粪便样本中的爱知株抗原方面很灵敏，但该ELISA与任何来源为肠道病毒、轮状病毒、诺沃克病毒、杯状病毒或星状病毒的非爱知株肠道病毒均无反应。在ELISA中，来自9起与牡蛎相关的肠胃炎疫情中5起的成年患者的47份粪便样本中有13份呈阳性，但在爱知县397份儿科粪便样本中只有1份呈阳性。发现7个月至4岁人群中爱知株抗体的流行率为7.2%。爱知株抗体的流行率随年龄增长而增加，35岁人群中约为80%。根据本研究结果，推测爱知株可能是一种新型小圆病毒，主要导致15至34岁年龄组患者腹泻，其中50%至76%的患者拥有中和抗体。
