Acerenza L
Department of Genetics, ICAPB, University of Edinburgh, U.K.
J Theor Biol. 1993 Nov 7;165(1):63-85. doi: 10.1006/jtbi.1993.1177.
The response of metabolic variables to small parameter changes is quantitatively described by the control coefficients. Their values (the control profile) depend on the rate equations of the enzymes which compose the metabolic system. A procedure, called Metabolic Control Design (MCD), is developed which achieves the "inverse", namely to calculate the kinetic properties of the enzymes which would produce a preconceived control profile when they are embedded in the network. It is shown that the lack of interaction between some variable metabolites and enzymes (i.e. some epsilon-elasticity coefficients are zero), together with the well-known conservation and summation relationships, reduce the number of control coefficients to which arbitrary values can be assigned. A choice of the values of these coefficients constitutes the pre-established control profile. The same procedure can also be used as an in situ method to detect unknown interactions between enzymes and metabolites. Finally, we discuss some implications of the results to the evolution of living organisms.
代谢变量对小参数变化的响应由控制系数定量描述。它们的值（控制图谱）取决于构成代谢系统的酶的速率方程。开发了一种称为代谢控制设计（MCD）的程序，该程序实现了“逆过程”，即计算当酶嵌入网络中时会产生预先设定的控制图谱的酶的动力学性质。结果表明，一些可变代谢物与酶之间缺乏相互作用（即一些ε弹性系数为零），再加上众所周知的守恒和求和关系，减少了可以赋予任意值的控制系数的数量。这些系数值的选择构成了预先设定的控制图谱。相同的程序也可以用作原位方法来检测酶与代谢物之间未知的相互作用。最后，我们讨论了这些结果对生物体进化的一些影响。
