Li Q, Colberg T R, Ownby C L
Department of Physiological Sciences, Oklahama State University, Stillwater 74078.
Toxicon. 1993 Sep;31(9):1197-201. doi: 10.1016/0041-0101(93)90135-6.
Many rattlesnake venoms (family Crotalidae) contain small, highly basic toxins which cause contracture and necrosis of skeletal muscle in experimental animals. Isolation of these myotoxins requires several chromatographic steps taking several days to complete. We report the isolation of crotamine, myotoxin a, and myotoxin a-like molecules from whole venom of three species of rattlesnakes using a Hydrazide Avidchrom Cartridge. Milligram amounts of highly purified and biologically active myotoxin can be obtained in 30 min to 2 hr.
许多响尾蛇毒液（蝰蛇科）含有小的、高度碱性的毒素，这些毒素会在实验动物体内引起骨骼肌挛缩和坏死。分离这些肌毒素需要几个色谱步骤，耗时数天才能完成。我们报告了使用酰肼亲和色谱柱从三种响尾蛇的全毒液中分离出响尾蛇胺、肌毒素a和肌毒素a样分子。在30分钟到2小时内可以获得毫克量的高度纯化且具有生物活性的肌毒素。
