Sambri V, Stefanelli C, Rossoni C, La Placa M, Cevenini R
Institute of Microbiology, St. Orsola Hospital, Bologna, Italy.
Appl Environ Microbiol. 1993 Nov;59(11):3938-40. doi: 10.1128/aem.59.11.3938-3940.1993.
Borrelia hermsii, Borrelia parkeri, Borrelia anserina, and Borrelia coriaceae produced several lipoproteins identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography of bacteria grown in [3H]palmitate. Five major acylated proteins were demonstrated by sequential alkaline and acid hydrolysis. High-pressure liquid chromatography of isolated proteins confirmed that covalently bound radioactivity was represented by fatty acids.
赫氏疏螺旋体、帕克疏螺旋体、鹅疏螺旋体和科氏疏螺旋体产生了几种脂蛋白，这些脂蛋白是通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳以及对在[3H]棕榈酸中生长的细菌进行荧光显影鉴定出来的。通过连续的碱水解和酸水解证明了五种主要的酰化蛋白。对分离出的蛋白质进行高压液相色谱分析证实，共价结合的放射性是由脂肪酸表示的。
