Lackey L W, Phelps T J, Bienkowski P R, White D C
Center for the Environmental Biotechnology, University of Tennessee-Knoxville 37996-2200.
Appl Biochem Biotechnol. 1993 Spring;39-40:701-13. doi: 10.1007/BF02919029.
Aliphatic chlorinated compounds, such as trichloroethylene (TCE) and tetrachloroethylene (PCE), are major contaminants of ground water. A single-pass packed-bed bioreactor was utilized to study the biodegradation of organic waste mixtures consisting of PCE, TCE, and other short-chain chlorinated organics. The bioreactor consisted of two 1960-mL glass columns joined in a series. One column was packed with sand containing a microbial consortia enriched from a contaminated site. The other column provided a reservoir for oxygen and a carbon source of methane/propane that was recirculated through the reactor. Sampling was accomplished by both direct headspace and liquid effluent concentration analyses. The reactor was operated in a single-pass mode. Greater than 99% degradation of trichloroethylene, approaching drinking water standards, was observed when the bioreactor residence time ranged from 1.9 to 3.2 d. Typically, when the reactor was pulse-fed with methane, propane, and air, 1 mol of TCE was degraded/110 mol of substrate utilized. Perturbation studies were performed to characterize reactor behavior. The system's degradation behavior was affected by providing different carbon sources, a pulse feeding regime, supplementing microbial biomass, and by altering flow rates.
脂肪族氯化化合物，如三氯乙烯（TCE）和四氯乙烯（PCE），是地下水的主要污染物。采用单通道填充床生物反应器研究了由PCE、TCE和其他短链氯化有机物组成的有机废物混合物的生物降解。该生物反应器由两个串联的1960毫升玻璃柱组成。一个柱填充有含有从污染场地富集的微生物群落的沙子。另一个柱为氧气和甲烷/丙烷碳源提供储存器，该碳源通过反应器再循环。通过直接顶空和液体流出物浓度分析完成采样。反应器以单通道模式运行。当生物反应器停留时间为1.9至3.2天时，观察到三氯乙烯的降解率大于99%，接近饮用水标准。通常，当向反应器脉冲供给甲烷、丙烷和空气时，每利用110摩尔底物降解1摩尔TCE。进行了扰动研究以表征反应器行为。通过提供不同的碳源、脉冲进料方式、补充微生物生物量以及改变流速，系统的降解行为受到影响。
