Scarselli E, Tolle R, Koita O, Diallo M, Müller H M, Früh K, Doumbo O, Crisanti A, Bujard H
Zentrum für Molekulare Biologie, Universität Heidelberg (ZMBH), Germany.
Infect Immun. 1993 Aug;61(8):3490-5. doi: 10.1128/iai.61.8.3490-3495.1993.
Thrombospondin-related anonymous protein (TRAP) of the malaria parasite Plasmodium falciparum shares two sequence motifs with other proteins which possess adhesive properties. Recently, findings indicate that TRAP is an antigen which contributes to antisporozoite immunity. We have cloned and expressed the TRAP coding sequences in Escherichia coli to investigate the human humoral immune response against this protein in a region of malaria endemicity of West Africa characterized by a seasonal transmission. Our results show that antibodies against TRAP are present in infected individuals. The anti-TRAP antibodies were analyzed in both a longitudinal and a prospective study. The longitudinal analysis shows seasonal fluctuations of the levels of specific antibodies as well as age-dependent quantitative differences. The immune response is long-lived in most of the adults and some of the older children but short-lived in young children. More importantly, the prospective analysis suggests that the presence of anti-TRAP antibodies in older children before the beginning of malaria transmission correlates with the subsequent control of parasite densities.
恶性疟原虫的血小板反应蛋白相关无名蛋白（TRAP）与其他具有黏附特性的蛋白质共有两个序列基序。最近的研究结果表明，TRAP是一种有助于抗子孢子免疫的抗原。我们已在大肠杆菌中克隆并表达了TRAP编码序列，以研究在西非疟疾流行地区季节性传播特征下人体针对该蛋白的体液免疫反应。我们的结果显示，感染个体中存在抗TRAP抗体。在纵向研究和前瞻性研究中均对抗TRAP抗体进行了分析。纵向分析显示特异性抗体水平存在季节性波动以及年龄依赖性的定量差异。大多数成年人和一些大龄儿童的免疫反应持久，但幼儿的免疫反应短暂。更重要的是，前瞻性分析表明，在疟疾传播开始之前大龄儿童中抗TRAP抗体的存在与随后对寄生虫密度的控制相关。
