Igarashi A, Okochi H, Bradham D M, Grotendorst G R
Department of Cell Biology and Anatomy, University of Miami School of Medicine, Florida 33136.
Mol Biol Cell. 1993 Jun;4(6):637-45. doi: 10.1091/mbc.4.6.637.
Connective tissue growth factor (CTGF) is a cysteine-rich peptide that exhibits platelet-derived growth factor (PDGF)-like biological and immunological activities. CTGF is a member of a family of peptides that include serum-induced immediate early gene products, a v-src-induced peptide, and a putative avian transforming gene, nov. In the present study, we demonstrate that human foreskin fibroblasts produce high levels of CTGF mRNA and protein after activation with transforming growth factor beta (TGF-beta) but not other growth factors including PDGF, epidermal growth factor, and basic fibroblast growth factor. Because of the high level selective induction of CTGF by TGF-beta, it appears that CTGF is a major autocrine growth factor produced by TGF-beta-treated human skin fibroblasts. Cycloheximide did not block the large TGF-beta stimulation of CTGF gene expression, indicating that it is directly regulated by TGF-beta. Similar regulatory mechanisms appear to function in vivo during wound repair where there is a coordinate expression of TGF-beta 1 before CTGF in regenerating tissue, suggesting a cascade process for control of tissue regeneration and repair.
结缔组织生长因子（CTGF）是一种富含半胱氨酸的肽，具有血小板衍生生长因子（PDGF）样的生物学和免疫学活性。CTGF是一个肽家族的成员，该家族包括血清诱导的即刻早期基因产物、v-src诱导肽以及一个假定的禽类转化基因nov。在本研究中，我们证明人包皮成纤维细胞在用转化生长因子β（TGF-β）激活后会产生高水平的CTGF mRNA和蛋白质，但在用包括PDGF、表皮生长因子和碱性成纤维细胞生长因子在内的其他生长因子激活后则不会。由于TGF-β对CTGF具有高水平的选择性诱导作用，因此CTGF似乎是TGF-β处理的人皮肤成纤维细胞产生的主要自分泌生长因子。放线菌酮并未阻断TGF-β对CTGF基因表达的强烈刺激，这表明它受TGF-β的直接调控。类似的调控机制似乎在伤口修复的体内过程中发挥作用，在再生组织中，TGF-β1在CTGF之前协同表达，这提示了一个控制组织再生和修复的级联过程。
