人羊水CA125和鳞状细胞癌抗原的来源

Origin of CA125 and SCC antigen in human amniotic fluid.

作者信息

Takeshima N, Suminami Y, Takeda O, Abe H, Kato H

机构信息

Department of Obstetrics and Gynecology, Yamaguchi University School of Medicine, Japan.

出版信息

Asia Oceania J Obstet Gynaecol. 1993 Jun;19(2):199-204. doi: 10.1111/j.1447-0756.1993.tb00373.x.

DOI:10.1111/j.1447-0756.1993.tb00373.x

PMID:8379869

Abstract

Levels of CA125 and SCC antigen in amniotic fluids were examined in 26 cases. All samples showed high levels of CA125 and SCC antigen. Statistically significant decreases in CA125 (p < 0.05) and increases in SCC antigen (p < 0.01) from the second to the third trimesters were recognized. We focused on amnion cells as likely sources of the 2 tumor markers in amniotic fluid. Analysis of in vitro culture of amnion cells and amniotic membranes revealed an accumulation of CA125 and no accumulation of SCC antigen in the culture supernatant. A Northern blot analysis using a cDNA probe of SCC antigen ensured that there was no mRNA expression of SCC antigen in the amnion, the cord or the placenta. It is likely that the amnion is a major source of CA125 in amniotic fluid, and that the fetus is the origin of SCC antigen.

摘要

对26例羊水样本检测了CA125和鳞状细胞癌抗原（SCC抗原）水平。所有样本的CA125和SCC抗原水平均较高。从孕中期到孕晚期，CA125出现具有统计学意义的下降（p < 0.05），SCC抗原出现具有统计学意义的上升（p < 0.01）。我们将羊膜细胞视为羊水中这两种肿瘤标志物可能的来源。对羊膜细胞和羊膜的体外培养分析显示，培养上清液中有CA125的积聚，而无SCC抗原的积聚。使用SCC抗原的cDNA探针进行的Northern印迹分析证实，羊膜、脐带或胎盘中不存在SCC抗原的mRNA表达。羊膜很可能是羊水中CA125的主要来源，而胎儿是SCC抗原的来源。