Aubin J T, Agut H, Collandre H, Yamanishi K, Chandran B, Montagnier L, Huraux J M
Laboratoire de Bactériologie-Virologie, C.E.R.V.I., Hôpital de la Pitié-Salpétrière, Paris, France.
J Virol Methods. 1993 Feb;41(2):223-34. doi: 10.1016/0166-0934(93)90129-f.
Ten human herpes virus 6 (HHV-6) strains from different origins were studied using reactivity to monoclonal antibodies and polymerase chain reaction analysis. Using immunofluorescence and neutralization assays, two monoclonal antibodies gave a positive reaction with the ten strains while three others only reacted with a fraction of these strains. This differential reactivity permitted segregation of the ten strains into two non-overlapping antigenic groups, designated as I and II. DNA was amplified from two regions of HHV-6 genome corresponding to the putative large tegument protein (LTP) gene and major capsid protein (MCP) gene, respectively. The restriction analysis of amplified products using HindIII for LTP and HaeII for MCP showed identical patterns among the strains belonging to the same antigenic group while BglII, TaqI and ClaI provided distinct patterns among group II strains. The nucleotide sequence of amplified products was determined and homology was found to be equal to or greater than 99% within each group whereas it was 96% between both groups. The number of amino-acid changes was higher when comparing two strains of different groups than when comparing two strains of the same group. The converging results of antigenic and genetic analyses led us to consider HHV-6 groups I and II as two distinct types of HHV-6 species.
利用针对单克隆抗体的反应性和聚合酶链反应分析，对来自不同来源的10株人疱疹病毒6型（HHV-6）毒株进行了研究。通过免疫荧光和中和试验，两种单克隆抗体与这10株毒株呈阳性反应，而另外三种仅与其中一部分毒株发生反应。这种不同的反应性使得这10株毒株可分为两个不重叠的抗原组，分别命名为I组和II组。分别从HHV-6基因组对应于假定的大被膜蛋白（LTP）基因和主要衣壳蛋白（MCP）基因的两个区域扩增DNA。使用HindIII对LTP进行扩增产物的限制性分析，使用HaeII对MCP进行分析，结果显示属于同一抗原组的毒株之间具有相同的图谱，而BglII、TaqI和ClaI在II组毒株之间提供了不同的图谱。测定了扩增产物的核苷酸序列，发现每组内的同源性等于或大于99%，而两组之间为96%。比较不同组的两株毒株时氨基酸变化的数量高于比较同一组的两株毒株时。抗原和遗传分析的趋同结果使我们将HHV-6的I组和II组视为HHV-6的两种不同类型。
