使用单克隆抗体通过酶联免疫吸附测定法鉴定9型人轮状病毒
Identification of serotype 9 human rotavirus by enzyme-linked immunosorbent assay with monoclonal antibodies.
作者信息
Midthun K, Valdesuso J, Kapikian A Z, Hoshino Y, Green K Y
机构信息
Laboratory of Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
出版信息
J Clin Microbiol. 1989 Sep;27(9):2112-4. doi: 10.1128/jcm.27.9.2112-2114.1989.
Abstract
Hybridomas producing monoclonal antibodies to VP7, a major neutralizing protein of serotype 9 rotavirus (strain W161), were prepared. One monoclonal antibody, W161-6A1, was shown to neutralize only serotype 9 rotavirus strains and reacted specifically with serotype 9 rotaviruses in an enzyme-linked immunosorbent assay. The development of an immunoassay for detection of serotype 9 rotaviruses should facilitate epidemiologic studies.
摘要
制备了产生针对9型轮状病毒（W161株）主要中和蛋白VP7的单克隆抗体的杂交瘤。一种单克隆抗体W161 - 6A1被证明仅能中和9型轮状病毒株，并且在酶联免疫吸附测定中与9型轮状病毒发生特异性反应。用于检测9型轮状病毒的免疫测定法的开发应有助于流行病学研究。
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