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Rapid serotyping of human rotavirus strains by solid-phase immune electron microscopy.通过固相免疫电子显微镜对人轮状病毒株进行快速血清分型
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Production and preliminary characterization of monoclonal antibodies directed at two surface proteins of rhesus rotavirus.针对恒河猴轮状病毒两种表面蛋白的单克隆抗体的制备及初步特性分析
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Direct isolation in cell culture of human rotaviruses and their characterization into four serotypes.人轮状病毒在细胞培养中的直接分离及其分为四种血清型的特性鉴定。
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Three human rotavirus serotypes demonstrated by plaque neutralization of isolated strains.通过对分离菌株进行蚀斑中和试验证明了三种人轮状病毒血清型。
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Definition of human rotavirus serotypes by plaque reduction assay.通过蚀斑减少试验对人轮状病毒血清型进行定义。
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Rotavirus serotypes by serum neutralisation.通过血清中和法检测的轮状病毒血清型
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Serotypic similarity and diversity of rotaviruses of mammalian and avian origin as studied by plaque-reduction neutralization.通过蚀斑减少中和试验研究的哺乳动物和禽类来源轮状病毒的血清型相似性和多样性
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Direct serotyping of human rotavirus in stools by an enzyme-linked immunosorbent assay using serotype 1-, 2-, 3-, and 4-specific monoclonal antibodies to VP7.通过使用针对VP7的1型、2型、3型和4型特异性单克隆抗体的酶联免疫吸附测定法对粪便中的人轮状病毒进行直接血清分型。
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Simple and specific enzyme immunoassay using monoclonal antibodies for serotyping human rotaviruses.使用单克隆抗体进行人轮状病毒血清分型的简单特异性酶免疫测定法。
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Single gene substitution rotavirus reassortants containing the major neutralization protein (VP7) of human rotavirus serotype 4.含有人类轮状病毒4型主要中和蛋白（VP7）的单基因替代轮状病毒重配株。
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使用单克隆抗体通过酶联免疫吸附测定法鉴定9型人轮状病毒

Identification of serotype 9 human rotavirus by enzyme-linked immunosorbent assay with monoclonal antibodies.

作者信息

Midthun K, Valdesuso J, Kapikian A Z, Hoshino Y, Green K Y

机构信息

Laboratory of Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.

出版信息

J Clin Microbiol. 1989 Sep;27(9):2112-4. doi: 10.1128/jcm.27.9.2112-2114.1989.

DOI:10.1128/jcm.27.9.2112-2114.1989
PMID:2550520
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC267751/
Abstract

Hybridomas producing monoclonal antibodies to VP7, a major neutralizing protein of serotype 9 rotavirus (strain W161), were prepared. One monoclonal antibody, W161-6A1, was shown to neutralize only serotype 9 rotavirus strains and reacted specifically with serotype 9 rotaviruses in an enzyme-linked immunosorbent assay. The development of an immunoassay for detection of serotype 9 rotaviruses should facilitate epidemiologic studies.

摘要

制备了产生针对9型轮状病毒（W161株）主要中和蛋白VP7的单克隆抗体的杂交瘤。一种单克隆抗体W161 - 6A1被证明仅能中和9型轮状病毒株，并且在酶联免疫吸附测定中与9型轮状病毒发生特异性反应。用于检测9型轮状病毒的免疫测定法的开发应有助于流行病学研究。