在传统培养管和空斑试验中使用MRC - 5细胞和A - 549细胞检测水痘带状疱疹病毒的比较

Comparison of MRC-5 and A-549 cells in conventional culture tubes and shell vial assays for the detection of varicella-zoster virus.

作者信息

Brinker J P, Doern G V

机构信息

Department of Clinical Microbiology, University of Massachusetts Medical Center, Worcester 01655.

出版信息

Diagn Microbiol Infect Dis. 1993 Jul;17(1):75-7. doi: 10.1016/0732-8893(93)90075-i.

DOI:10.1016/0732-8893(93)90075-i

PMID:8395374

Abstract

A-549 and MRC-5 cells were compared for the detection of varicella-zoster virus (VZV) by conventional tube cell culture and by shell vial assay using fresh specimens. VZV was detected in 33 (32.4%) of 102 specimens by one or all of these methods. In conventional tube culture, seven (21.2%) of 33 were positive in MRC-5 cells and 24 (72.7%) of 33 were positive in A-549 cells, a threefold increase in sensitivity. In shell vial assay, 30 (90.9%) of 33 were positive in MRC-5 cells and 31 (93.9%) of 33 were positive in A-549 cells. The samples tested by A-549 shell vial assay had more positives showing plaque formation. The two shell vial procedures gave the highest levels of sensitivity. A-549 cells provided a more sensitive method of detecting VZV in clinical specimens in both conventional tubes and shell vial assays than did MRC-5 cells.

摘要

采用传统的试管细胞培养法和使用新鲜标本的空斑试验，对A-549细胞和MRC-5细胞检测水痘带状疱疹病毒（VZV）的能力进行了比较。通过这些方法中的一种或全部，在102份标本中的33份（32.4%）检测到了VZV。在传统的试管培养中，33份中的7份（21.2%）在MRC-5细胞中呈阳性，33份中的24份（72.7%）在A-549细胞中呈阳性，敏感性提高了三倍。在空斑试验中，33份中的30份（90.9%）在MRC-5细胞中呈阳性，33份中的31份（93.9%）在A-549细胞中呈阳性。经A-549空斑试验检测的样本中有更多阳性显示有噬斑形成。两种空斑试验方法的敏感性最高。在传统试管培养和空斑试验中，与MRC-5细胞相比，A-549细胞为检测临床标本中的VZV提供了一种更敏感的方法。