Bitterman N, Bitterman H, Kinarty A, Melamed Y, Lahat N
Israeli Naval Hyperbaric Institute, Haifa.
Undersea Hyperb Med. 1993 Sep;20(3):197-204.
We studied the effect of a single exposure to a therapeutic profile of hyperbaric oxygen on blood mononuclear cell subset. Twenty healthy volunteers were exposed to 0.28 MPa for 90 min. Thirteen breathed pure oxygen and seven were control subjects exposed to compressed air at the same pressure. Venous blood samples were drawn before HBO exposure, immediately on exit from the chamber, and 24 h later. Immediately after the exposure, a significant increase was observed in the percentage and absolute number of CD8 (suppressor/cytotoxic) T cells, with a concomitant decrease in the CD4 (helper/inducer) T cells. These changes resulted in a decreased CD4:CD8 ratio. A rise was also observed in the number of HLA-DR antigen-bearing cells, with a transient increase in monocytes. There was no change in the total count and percentage of T cells (CD3), B cells, and NK cells. Twenty-four hours after HBO exposure there was a partial reversal of the decrease in the mean CD4:CD8 ratio, but it was still significantly lower than preexposure values. The fast reversibility of the change in the CD4:CD8 ratio suggests specific HBO-induced shifts and sequestration of T-cell subpopulations.
我们研究了单次暴露于具有治疗作用的高压氧对血液单核细胞亚群的影响。20名健康志愿者暴露于0.28兆帕的环境中90分钟。其中13人呼吸纯氧，7人作为对照受试者在相同压力下暴露于压缩空气中。在高压氧暴露前、出舱后即刻以及24小时后采集静脉血样本。暴露后即刻，观察到CD8（抑制/细胞毒性）T细胞的百分比和绝对数量显著增加，同时CD4（辅助/诱导）T细胞减少。这些变化导致CD4:CD8比值降低。还观察到携带HLA-DR抗原的细胞数量增加，单核细胞短暂增多。T细胞（CD3）、B细胞和NK细胞的总数及百分比没有变化。高压氧暴露24小时后，平均CD4:CD8比值下降部分逆转，但仍显著低于暴露前的值。CD4:CD8比值变化的快速可逆性表明高压氧特异性诱导了T细胞亚群的转移和隔离。
