Lahat N, Bitterman H, Yaniv N, Kinarty A, Bitterman N
Israel Naval Medical Institute, Immunological Research Unit, Haifa, Israel.
Clin Exp Immunol. 1995 Dec;102(3):655-9. doi: 10.1111/j.1365-2249.1995.tb03867.x.
We investigated the secretion of TNF-alpha by monocytes and macrophages derived from the peripheral blood, spleen, and lungs after a single exposure to a therapeutic profile of hyperbaric oxygen (HBO). Rats were exposed for 90 min to either 100% oxygen at 0.28 MPa (2.8 atmospheres absolute) or air. Immediately after exposure, mononuclear cells were isolated from blood, spleen, and lungs and cultured for 18 h. The secretion of TNF-alpha from the cultured monocytes/macrophages was determined with and without stimulation with lipopolysaccharide (LPS). Exposure to hyperbaric oxygen induced a significant increase in the spontaneous ex vivo secretion of TNF-alpha (without LPS) by mononuclear cells from the blood, spleen, and lung (P < 0.05 from air controls). Stimulation with LPS after exposure to HBO induced a significant increase in TNF-alpha secretion by lung and spleen macrophages compared with air controls (P < 0.05). However, absolute TNF-alpha levels were not significantly higher than those achieved 'spontaneously' in macrophages exposed to HBO without LPS. Stimulation with LPS induced a marked increase in secretion of TNF-alpha from blood monocytes after exposure to air, but not after exposure to HBO. These results provide evidence in support of a role played by TNF-alpha in mediating HBO effects on different tissues and their immune responses.
我们研究了单次暴露于高压氧（HBO）治疗参数后，外周血、脾脏和肺脏来源的单核细胞和巨噬细胞中肿瘤坏死因子-α（TNF-α）的分泌情况。将大鼠暴露于0.28 MPa（2.8个绝对大气压）的100%氧气或空气中90分钟。暴露后立即从血液、脾脏和肺脏中分离单核细胞并培养18小时。在有或无脂多糖（LPS）刺激的情况下，测定培养的单核细胞/巨噬细胞中TNF-α的分泌。暴露于高压氧会导致血液、脾脏和肺脏的单核细胞在体外自发分泌TNF-α（无LPS）显著增加（与空气对照组相比，P < 0.05）。暴露于HBO后用LPS刺激，与空气对照组相比，肺脏和脾脏巨噬细胞中TNF-α的分泌显著增加（P < 0.05）。然而，TNF-α的绝对水平并不显著高于未用LPS处理的暴露于HBO的巨噬细胞“自发”达到的水平。用LPS刺激会使暴露于空气后的血液单核细胞中TNF-α的分泌显著增加，但暴露于HBO后则不会。这些结果为TNF-α在介导HBO对不同组织及其免疫反应的作用提供了证据支持。
