Fry J M, Smith G M, McGrath M C, Speijers E J, Allen J G
Animal Health Division, Western Australian Department of Agriculture, Baron Hay-court, South Perth.
Br J Nutr. 1993 Jan;69(1):225-32. doi: 10.1079/bjn19930024.
To determine the relationship between plasma and tissue alpha-tocopherol concentrations during vitamin E depletion, weaned lambs were placed on a vitamin E-deficient diet for 0, 1, 2, 4, 8 and 12 weeks. alpha-Tocopherol was measured in plasma, erythrocytes, liver, adrenal, adipose tissue, three different skeletal muscles and heart muscle. The alpha-tocopherol concentration in plasma fell at the same rate as the alpha-tocopherol concentration in skeletal muscles, heart muscle, adrenal and adipose tissue. The alpha-tocopherol concentration in liver and erythrocytes fell at a faster rate than that of plasma and all muscle tissues. There were significant correlations between alpha-tocopherol concentration in plasma and alpha-tocopherol concentrations in all the tissues measured. Different skeletal muscles had significantly different concentrations of alpha-tocopherol which may relate to their differing susceptibility to nutritional myopathy. The increase in malondialdehyde in oxidatively-stressed muscle tissue and the correlation with alpha-tocopherol concentration in most muscle tissues indicated that the muscles had reduced antioxidant capacity in vitro as a result of vitamin E depletion. It was concluded that during vitamin E depletion in sheep alpha-tocopherol concentration in plasma was a good index of vitamin E status under the experimental conditions employed.
为了确定维生素E缺乏期间血浆和组织中α-生育酚浓度之间的关系，将断奶羔羊置于维生素E缺乏的饮食中0、1、2、4、8和12周。测量血浆、红细胞、肝脏、肾上腺、脂肪组织、三种不同的骨骼肌和心肌中的α-生育酚。血浆中α-生育酚浓度下降的速率与骨骼肌、心肌、肾上腺和脂肪组织中α-生育酚浓度下降的速率相同。肝脏和红细胞中α-生育酚浓度下降的速率比血浆和所有肌肉组织更快。血浆中α-生育酚浓度与所测量的所有组织中的α-生育酚浓度之间存在显著相关性。不同的骨骼肌中α-生育酚浓度存在显著差异，这可能与其对营养性肌病的不同易感性有关。氧化应激肌肉组织中丙二醛的增加以及与大多数肌肉组织中α-生育酚浓度的相关性表明，由于维生素E缺乏，肌肉在体外的抗氧化能力降低。得出的结论是，在绵羊维生素E缺乏期间，在所采用的实验条件下，血浆中α-生育酚浓度是维生素E状态的良好指标。
