Tanaka S, Isono K
Postgraduate School of Science and Technology, Kobe University, Japan.
Nucleic Acids Res. 1993 Mar 11;21(5):1149-53. doi: 10.1093/nar/21.5.1149.
Most of the 155 transcripts of the genes on chromosome III of S.cerevisiae that were identified by a series of Northern hybridization experiments (Yoshikawa, A. and Isono, K., Yeast 6, 383-401, 1990) have been correlated with the open reading frames (ORFs) deduced from the nucleotide sequence data for this chromosome (Oliver, S.G. et al., Nature 357, 38-46, 1992). This was performed by comparing the experimentally constructed physical map and the one produced from the nucleotide sequence data, and then the sizes and positions of observed transcripts and those of sequenced ORFs. The data thus provide information for possible products of the sequenced ORFs (genes) of chromosome III of S.cerevisiae as well as their likely levels of expression in vegetatively growing cells.
通过一系列Northern杂交实验鉴定出的酿酒酵母III号染色体上155个基因的转录本(吉川,A.和矶野,K.,《酵母》6,383 - 401,1990),大多数已与根据该染色体核苷酸序列数据推导的开放阅读框(ORF)相关联(奥利弗,S.G.等人,《自然》357,38 - 46,1992)。这是通过比较实验构建的物理图谱和由核苷酸序列数据生成的图谱,然后比较观察到的转录本与测序ORF的大小和位置来完成的。这些数据因此为酿酒酵母III号染色体测序ORF(基因)的可能产物及其在营养生长细胞中的可能表达水平提供了信息。