Noiri Y, Ozaki K, Nakae H, Matsuo T, Ebisu S
Department of Conservative Dentistry, Osaka University, Faculty of Dentistry, Japan.
J Periodontal Res. 1997 Oct;32(7):598-607. doi: 10.1111/j.1600-0765.1997.tb00937.x.
The localization and distribution of Porphyromonas gingivalis, Campylobacter rectus and Actinomyces viscosus were studied in human periodontal pockets. After obtaining voluntary consent from 9 patients, 12 teeth and their surrounding periodontal tissue with advanced adult periodontitis were extracted carefully so as not to change the structure of the periodontal pockets. The specimens were processed into serial sections. One of the sections was stained with Brown & Brenn-modified Gram stain to observe the distribution of bacteria. The others were stained immunohistochemically by the Labelled Streptavidin Biotin method (LSAB method) using specific rabbit antibodies against selected bacteria. Some bacteria could be found within epithelial cells. P. gingivalis was found in 9/12 of the samples examined. Small aggregates of P. gingivalis were scattered in all parts of the periodontal pockets, and some of these aggregates could be seen in close contact with the epithelium. Conversely, C. rectus was observed in 5/12 of the samples examined and was predominantly located in the middle and deep pocket zones. C. rectus tended to form large clumps in both the tooth-attached and epithelium-associated plaque area. A. viscosus was observed in 7/12 of the samples examined and was localized predominantly in the tooth-attached plaque area, especially in the shallow and middle pocket zones. Although unexpected spills of unattached plaque from periodontal pockets was possible, immunohistochemical staining with species-specific antibodies was extremely sensitive and revealed the localization and the distribution of periodontal disease-associated bacteria in human periodontal pockets.
对牙龈卟啉单胞菌、直肠弯曲菌和粘性放线菌在人类牙周袋中的定位和分布进行了研究。在获得9名患者的自愿同意后，仔细拔除了12颗患有晚期成人牙周炎的牙齿及其周围的牙周组织，以免改变牙周袋的结构。将标本制成连续切片。其中一张切片用改良的Brown & Brenn革兰氏染色法染色，以观察细菌的分布。其他切片则采用标记链霉亲和素生物素法（LSAB法），使用针对特定细菌的特异性兔抗体进行免疫组织化学染色。在上皮细胞内可发现一些细菌。在所检查的12个样本中，有9个样本检测到牙龈卟啉单胞菌。牙龈卟啉单胞菌的小聚集体散在于牙周袋的各个部位，其中一些聚集体可见与上皮紧密接触。相反，在所检查的12个样本中，有5个样本检测到直肠弯曲菌，其主要位于牙周袋的中部和深部区域。直肠弯曲菌倾向于在牙附着菌斑区和上皮相关菌斑区形成大的菌团。在所检查的12个样本中，有7个样本检测到粘性放线菌，其主要定位于牙附着菌斑区，尤其是在牙周袋的浅部和中部区域。尽管牙周袋中未附着菌斑可能意外溢出，但使用种特异性抗体进行免疫组织化学染色极其敏感，揭示了人类牙周袋中与牙周病相关细菌的定位和分布。
