Bergez P, Doignon F, Crouzet M
Laboratoire de Biologie Moléculaire et de Séquençage, UPR CNRS 9026, Université de Bordeaux II, France.
Yeast. 1995 Aug;11(10):967-74. doi: 10.1002/yea.320111008.
We have determined the complete nucleotide sequence of a 44 420 bp DNA fragment from chromosome XIV of Saccharomyces cerevisiae. The sequence data revealed 23 open reading frames (ORFs) larger than 300 bp, covering 73.5% of the sequence. The ORFs N2418, N2428, N2441, N2474 and N2480 correspond to previously sequenced S. cerevisiae genes coding respectively for the mitochondrial import protein Mas5, the nucleolar protein Nop2, the outer mitochondrial membrane porin Por1, the cytochrome c oxidase polypeptide VA precursor CoxA and the yeast protein tyrosine phosphatase Msg5. Translation products of three other ORFs N2406, N2411 and N2430 exhibit similarity to previously known S. cerevisiae proteins: the ribosomal protein YL9A, the protein Nca3 involved in the mitochondrial expression of subunits 6 and 8 of the ATP synthase and actin; in addition N2505 presents strong similarity to an ORF of chromosome IX. The predicted protein products of ORFs N2417 and N2403 present similarities with domains from proteins of other organisms: the Candida maltosa cycloheximide-resistance protein, the human interleukin enhancer-binding factor (ILF-2). The 12 remaining ORFs show no significant similarity to known proteins. In addition, we have detected a DNA region very similar to the yeast transposon Ty 1-15 of which insertion has disrupted a tRNA(Asp) gene.
我们已经确定了来自酿酒酵母第十四号染色体的一个44420 bp DNA片段的完整核苷酸序列。序列数据显示有23个大于300 bp的开放阅读框（ORF），覆盖了该序列的73.5%。ORF N2418、N2428、N2441、N2474和N2480分别对应于先前测序的酿酒酵母基因，它们编码线粒体导入蛋白Mas5、核仁蛋白Nop2、线粒体外膜孔蛋白Por1、细胞色素c氧化酶多肽VA前体CoxA和酵母蛋白酪氨酸磷酸酶Msg5。其他三个ORF N2406、N2411和N2430的翻译产物与先前已知的酿酒酵母蛋白具有相似性：核糖体蛋白YL9A、参与ATP合酶亚基6和8线粒体表达的蛋白Nca3以及肌动蛋白；此外，N2505与九号染色体的一个ORF具有很强的相似性。ORF N2417和N2403的预测蛋白产物与其他生物体蛋白的结构域具有相似性：麦芽糖假丝酵母环己酰亚胺抗性蛋白、人白细胞介素增强子结合因子（ILF - 2）。其余12个ORF与已知蛋白没有明显的相似性。此外，我们检测到一个与酵母转座子Ty 1 - 15非常相似的DNA区域，其插入破坏了一个tRNA（Asp）基因。
