Shigematsu M, Meno Y, Misumi H, Amako K
Department of Bacteriology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Microbiol Immunol. 1995;39(10):741-4. doi: 10.1111/j.1348-0421.1995.tb03260.x.
The swimming velocities of two monotrichous flagellated bacteria were measured by a computer-assisted video tracking method. Tracing the moving path of the individual bacterium revealed that the bacterial cell did not swim continuously in a straight direction, but frequently changed swimming direction and velocity. The average swimming velocities calculated from the 3-sec path were 75.4 +/- 9.4 microns/sec in four strains of Vibrio cholerae and 51.3 +/- 8.4 microns/sec in five strains of Pseudomonas aeruginosa. These results suggest that V. cholerae swim faster than P. aeruginosa at 30 C in nutrient broth. This method is useful for a detailed analysis of bacterial movement and moving patterns in different environmental conditions.
通过计算机辅助视频跟踪方法测量了两种单端鞭毛菌的游动速度。追踪单个细菌的移动路径发现，细菌细胞并非沿直线持续游动，而是频繁改变游动方向和速度。根据3秒路径计算出的四株霍乱弧菌的平均游动速度为75.4±9.4微米/秒，五株铜绿假单胞菌的平均游动速度为51.3±8.4微米/秒。这些结果表明，在30℃的营养肉汤中，霍乱弧菌比铜绿假单胞菌游动得更快。该方法有助于详细分析不同环境条件下细菌的运动和移动模式。
