Yan R Q, Su J J, Huang D R, Gan Y C, Yang C, Huang G H
Department of Pathology, Guangxi Institute for Research on Cancer, Nanning, P. R. China.
J Cancer Res Clin Oncol. 1996;122(5):283-8. doi: 10.1007/BF01261404.
Tree shrews (Tupaia belangeri chinenesis) can be experimentally infected with human hepatitis B virus (HBV) by inoculation with human serum positive for HBV, the experimental infection rate being 55.21%. Successive infections have been passed through five generations among the tree shrews inoculated with HBV-positive sera from the infected animals, the average infection rate being 94.0%. The experimental infection of tree shrews with HBV may be prevented by immunization with hepatitis B vaccine, the protection rate being 88.89%. Standard serum containing HBV at 10(8) CID (chimpanzee infection dose)/ml, was diluted 10(-6), 10(-7), 10(-8), 10(-9), and 10(-10) and produced infection rates of 80.0%, 88.8%, 66.7%, 55.6% and 42.9% respectively. Thus the CID50 in tree shrews may reach a dilution of 10(-9), which shows that tree shrews are sensitive to HBV infection. These results successfully establish tree shrews as a reliable and useful animal model for research on HBV infection and its relation to hepatocarcinogenesis.
树鼩（Tupaia belangeri chinenesis）可通过接种乙肝病毒阳性人血清而被实验性感染乙肝病毒（HBV），实验感染率为55.21%。在接种来自受感染动物的乙肝病毒阳性血清的树鼩中，连续感染已传至五代，平均感染率为94.0%。用乙肝疫苗免疫可预防树鼩被乙肝病毒实验性感染，保护率为88.89%。将含10(8) CID（黑猩猩感染剂量）/毫升乙肝病毒的标准血清分别稀释10(-6)、10(-7)、10(-8)、10(-9)和10(-10)，产生的感染率分别为80.0%、88.8%、66.7%、55.6%和42.9%。因此，树鼩的半数感染剂量（CID50）可能达到10(-9)的稀释度，这表明树鼩对乙肝病毒感染敏感。这些结果成功地确立了树鼩作为研究乙肝病毒感染及其与肝癌发生关系的可靠且有用的动物模型。
