Andersen B M
Dept. of Hospital Infections, Clinic for Preventive Medicine, Ullevål University Hospital, Oslo, Norway.
Infection. 1995 Nov-Dec;23(6):339-43. doi: 10.1007/BF01713562.
During a period of 2 years, 118 strains of Enterobacter cloacae were collected consecutively in connection with nosocomial infections in Northern Norway; identified by conventional methods and by the API 20E system. The API 20E profile 3305573 predominated and was present in 73 of 118 strains. Among 96 serotyped strains, 73 were serotypable, 20 nontypable and two polyagglutinable. Predominating serotypes were 3 (29 strains), 8 (21 strains) and 23 (nine strains). When the API 20E profiles of the 118 strains were read in the new ATB (automated computer-assisted) 20E data base system, 97 of 118 (82.2%) strains were identified as E. cloacae. The 118 strains were tested in the new ATB Rapid ID 32E and ATB ID 32E (ATB system, bioMérieux, France) systems. Only 69 of 118 (58.5%) strains were identified as E. cloacae in both systems. The ATB Rapid ID 32E identified 97 of 118 strains (82.2%), and the ATB ID 32E only 80 of 118 strains (67.8%). Among 73 serotypable strains, the ATB Rapid ID 32E identified 79.5% as E. cloacae, while the ATB ID 32E identified only 64.4%. Among 40 serotypable strains with API 20E profile 3305573, all 40 were identified as E. cloacae by the ATB Rapid ID 32E, while only 27 (67.5%) by the ATB ID 32E system. Further improvements may increase the value of biochemical identification of E. cloacae in diagnostic work.
在两年时间里，从挪威北部医院感染病例中连续收集了118株阴沟肠杆菌；采用传统方法和API 20E系统进行鉴定。API 20E谱型3305573占主导地位，在118株菌株中有73株呈现该谱型。在96株进行血清分型的菌株中，73株可进行血清分型，20株无法分型，2株呈现多凝集性。主要血清型为3型（29株）、8型（21株）和23型（9株）。当在新的ATB（自动计算机辅助）20E数据库系统中读取这118株菌株的API 20E谱型时，118株中有97株（82.2%）被鉴定为阴沟肠杆菌。这118株菌株在新的ATB Rapid ID 32E和ATB ID 32E（ATB系统，法国生物梅里埃公司）系统中进行了检测。在这两个系统中，118株中只有69株（58.5%）被鉴定为阴沟肠杆菌。ATB Rapid ID 32E鉴定出118株中的97株（82.2%），而ATB ID 32E仅鉴定出118株中的80株（67.8%）。在73株可进行血清分型的菌株中，ATB Rapid ID 32E将79.5%鉴定为阴沟肠杆菌，而ATB ID 32E仅鉴定出64.4%。进一步改进可能会提高阴沟肠杆菌生化鉴定在诊断工作中的价值。
