Fahrenkrug J, Schaffalitzky de Muckadell O B, Holst J J
Scand J Gastroenterol. 1977;12(3):273-9. doi: 10.3109/00365527709180928.
The concentration of immunoreactive secretin in portal blood and the secretion from the exocrine pancreas were measured during intraduodenal infusion of isotonic or hypertonic saline, isotonic or hypertonic glucose, aminoacids, fat emulsion, or 0.1 mol X 1(-1) hydrochloric acid in 7 anaesthetized pigs. None of these substances, except hydrochloric acid, had any effect on plasma secretin concentration and pancreatic flow rate and bicarbonate output. Plasma secretin concentration rose significantly from 5.6 +/- 2.7 pmol X 1(-1) (mean +/- S.E.M.) to a peak value of 201.2 +/- 80.5 pmol X 1(-1) 15 min after infusion of hydrochloric acid. Pancreatic flow rate and bicarbonate output increased from 0.51 +/- 0.19 ml X h-1 (mean +/- S.E.M.) to 9.85 +/- 2.33 ml X h-1 and from 52 +/- 11 micronmol X h-1 to 1.004 +/- 290 micronmol X h-1, respectively. During intraduodenal introduction of meals with pH adjusted from 1.0 to 7.0 in 4 pigs amylase was secreted at all pH levels. However, only when pH of the meal was 1.0, resulting in an intraduodenal pH from 1.0 to 1.7 during the stimulation, was a significant increase in plasma secretin concentration and pancreatic flow rate observed from 5.5 +/- 2.8 pmol X 1(-1) (mean +/- S.E.M.) to 115.0 +/- 51.2 pmol X 1(-1) and from 0.20 +/- 0.08 ml X h-1 to 6.25 +/- 2.57 ml X h-1, respectively.
在7只麻醉猪中，于十二指肠内输注等渗或高渗盐水、等渗或高渗葡萄糖、氨基酸、脂肪乳剂或0.1mol/L盐酸期间，测定了门静脉血中免疫反应性促胰液素的浓度以及外分泌胰腺的分泌情况。除盐酸外，这些物质均未对血浆促胰液素浓度、胰腺血流量和碳酸氢盐输出产生任何影响。输注盐酸15分钟后，血浆促胰液素浓度从5.6±2.7pmol/L（平均值±标准误）显著升至峰值201.2±80.5pmol/L。胰腺血流量和碳酸氢盐输出分别从0.51±0.19ml/h（平均值±标准误）增至9.85±2.33ml/h，以及从52±11μmol/h增至1.004±290μmol/h。在4只猪中，当十二指肠内引入pH值从1.0调整至7.0的餐食时，在所有pH水平下均有淀粉酶分泌。然而，仅当餐食pH值为1.0，刺激期间十二指肠内pH值为1.0至1.7时，才观察到血浆促胰液素浓度和胰腺血流量显著增加，分别从5.5±2.8pmol/L（平均值±标准误）增至115.0±51.2pmol/L，以及从0.20±0.08ml/h增至6.25±2.57ml/h。
