Benaissa M, Babin P, Quellard N, Pezennec L, Cenatiempo Y, Fauchère J L
Laboratoire de Microbiologie A, Centre Hospitalier Regional Universitaire-La Miletrie, Poitiers, France.
Infect Immun. 1996 Jun;64(6):2331-5. doi: 10.1128/iai.64.6.2331-2335.1996.
In vitro, Helicobacter pylori converts from a bacillary to a full coccoid form via an intermediate U-shaped form. Organisms with a full coccoid form keep a double membrane system, a polar membrane, and invagination structures. Western blots (immunoblots) of sera from colonized patients show that some high-molecular-mass antigenic fractions are expressed only in coccoids. Conversely, fractions of 30 and 94 kDa were more intensively detected in the bacillary forms. These results suggest that (i) coccoid conversion is not a degenerative transformation and (ii) antigens specific to the coccoid forms are expressed in vivo.
在体外，幽门螺杆菌通过中间的U形形态从杆菌形态转变为完全球菌形态。具有完全球菌形态的生物体保留了双膜系统、极性膜和内陷结构。对定植患者血清进行的蛋白质免疫印迹分析表明，一些高分子量抗原组分仅在球菌中表达。相反，在杆菌形态中更强烈地检测到30 kDa和94 kDa的组分。这些结果表明：（i）球菌转化不是一种退化性转变；（ii）球菌形态特异性抗原在体内表达。
