Schaeberle M D, Kalasinsky V F, Luke J L, Lewis E N, Levin I W, Treado P J
Department of Chemistry, University of Pittsburgh, Pennsylvania 15260, USA.
Anal Chem. 1996 Jun 1;68(11):1829-33. doi: 10.1021/ac951245a.
High-definition Raman chemical imaging microscopy is applied to the histopathological characterization of biopsied human breast tissue containing foreign polymer inclusions. The polymer material is found in a patient with a history of silicone implant breast reconstructive surgery. Silicone implants are, on occasion, anchored to the soft tissues of the chest with polyester patches. In the case studied here, the polyester anchors were incorporated into the patient's fibrous tissue surrounding the implant. High-definition Raman chemical imaging provides molecular-specific image contrast in the complex breast tissue matrix without the use of stains or dyes. This is the first example in which Raman spectroscopic imaging microscopy has been applied to pathology. A goal of this and future studies is to complement traditional histopathologic diagnoses of disease states utilizing vibrational spectroscopic imaging techniques.
高清拉曼化学成像显微镜被应用于对含有外来聚合物包涵体的活检人类乳腺组织进行组织病理学特征分析。在一名有硅胶植入乳房重建手术史的患者体内发现了这种聚合物材料。硅胶植入物有时会用聚酯贴片固定在胸部软组织上。在本研究的病例中，聚酯锚定物被纳入患者植入物周围的纤维组织中。高清拉曼化学成像在不使用染色剂或染料的情况下，能在复杂的乳腺组织基质中提供分子特异性图像对比度。这是拉曼光谱成像显微镜应用于病理学的首个实例。本研究及未来研究的一个目标是利用振动光谱成像技术辅助疾病状态的传统组织病理学诊断。
