Mao Y, Nobaek S, Kasravi B, Adawi D, Stenram U, Molin G, Jeppsson B
Department of Surgery, Lund University, Sweden.
Gastroenterology. 1996 Aug;111(2):334-44. doi: 10.1053/gast.1996.v111.pm8690198.
BACKGROUND & AIMS: Administration of methotrexate to rats on an elemental diet results in severe enterocolitis and death. Lactobacilli, an integral part of the healthy gastrointestinal microecology, may provide therapeutic benefits to help the recovery from enterocolitis. The purpose of this study was to evaluate the effects of lactobacilli and oatbase on methotrexate-induced enterocolitis in rats.
Rats received continuous intragastric infusion of elemental diet or with supplementation of oatbase, Lactobacillus reuteri R2LC, and Lactobacillus plantarum DSM 9843, with and without fermentation, from the beginning of the study. Methotrexate (20 mg/kg) was injected intraperitoneally on day 3, and the sampling was performed on day 6.
Lactobacilli and oatbase decreased body weight loss and intestinal permeability and increased bowel mucosal mass in enterocolitic rats. Administration of lactobacilli, but not oatbase, decreased the intestinal myeloperoxidase level, reestablished intestinal microecology, and reduced bacterial translocation to extraintestinal sites. Both lactobacilli and oatbase reduced plasma endotoxin levels. The effects of lactobacilli were greater with fermentation than without fermentation or oatbase alone, and L. plantarum was more effective in reducing intestinal pathogens than L. reuteri.
Exogenous administration of lactobacilli, especially L. plantarum with fermentation, is helpful in reducing the severity of enterocolitis in rats.
给食用要素饮食的大鼠注射甲氨蝶呤会导致严重的小肠结肠炎并死亡。乳酸杆菌作为健康胃肠道微生态的一个组成部分，可能具有治疗作用，有助于小肠结肠炎的恢复。本研究旨在评估乳酸杆菌和燕麦粉对甲氨蝶呤诱导的大鼠小肠结肠炎的影响。
从研究开始，大鼠持续接受胃内输注要素饮食，或补充燕麦粉、罗伊氏乳杆菌R2LC和植物乳杆菌DSM 9843，有无发酵处理。在第3天腹腔注射甲氨蝶呤（20 mg/kg），并在第6天进行采样。
乳酸杆菌和燕麦粉可减轻小肠结肠炎大鼠的体重减轻和肠道通透性，并增加肠黏膜质量。给予乳酸杆菌（而非燕麦粉）可降低肠道髓过氧化物酶水平，重建肠道微生态，并减少细菌易位至肠外部位。乳酸杆菌和燕麦粉均可降低血浆内毒素水平。乳酸杆菌发酵后的效果比未发酵或单独使用燕麦粉更好，且植物乳杆菌在减少肠道病原体方面比罗伊氏乳杆菌更有效。
外源性给予乳酸杆菌，尤其是发酵后的植物乳杆菌，有助于减轻大鼠小肠结肠炎的严重程度。
