Grundy J E, Ehrnst A, Einsele H, Emery V C, Hebart H, Prentice H G, Ljungman P
Department of Clinical Immunology, Royal Free Hospital School of Medicine, London, United Kingdom.
J Clin Microbiol. 1996 May;34(5):1166-70. doi: 10.1128/jcm.34.5.1166-1170.1996.
The presence of cytomegalovirus (CMV) in the blood has important consequences for patient management, and an external quality control study of its detection by the PCR was conducted by the Infectious Disease Working Party of the European Group for Blood and Marrow Transplantation. Forty-eight coded peripheral blood samples from bone marrow transplant recipients were processed in parallel in three European centers by using the routine in-house PCR assay. Protocols varied in choice of primers, specificity and amplificability controls, and sample processing. Results for 38 of 47 samples agreed, 35 being negative and 3 positive. Of the 12 samples reported as positive by a least one center, only 3 were found to be positive by all three centers, 1 was found to be positive by two centers, and the remaining 8 were found to be positive by one center only. The nine discrepant samples appeared to contain around 1,000-fold less viral DNA than the three concordant positive samples. CMV detection was affected both by the number of leukocytes from which DNA was extracted and by the number of cell equivalents added per PCR. External quality control schemes for CMV PCR are clearly necessary in order to compare data from different centers, and recommendation for standardizing the PCR detection of CMV in blood leukocytes are made.
血液中巨细胞病毒（CMV）的存在对患者管理具有重要影响，欧洲血液和骨髓移植组传染病工作组开展了一项关于通过聚合酶链反应（PCR）检测CMV的外部质量控制研究。来自骨髓移植受者的48份编码外周血样本在三个欧洲中心采用常规内部PCR检测方法进行平行处理。各中心在引物选择、特异性和扩增性对照以及样本处理方面的方案各不相同。47份样本中有38份结果一致，35份为阴性，3份为阳性。在至少一个中心报告为阳性的12份样本中，只有3份在所有三个中心均呈阳性，1份在两个中心呈阳性，其余8份仅在一个中心呈阳性。这9份结果不一致的样本所含病毒DNA似乎比3份结果一致的阳性样本少约1000倍。CMV检测受提取DNA的白细胞数量以及每个PCR反应中加入的细胞当量数量的影响。显然有必要实施CMV PCR外部质量控制方案，以便比较不同中心的数据，并对血液白细胞中CMV的PCR检测标准化提出建议。
