Petney T N, Hassler D, Brückner M, Maiwald M
Department of Parasitology, University of Heidelberg, Germany.
J Clin Microbiol. 1996 May;34(5):1310-2. doi: 10.1128/jcm.34.5.1310-1312.1996.
PCR was used to compare urinary bladder and ear biopsy samples from four European species of wild rodents for the presence of Borrelia burgdorferi sensu lato. From 60 paired comparisons of bladder and ear biopsy samples, the PCR results were concordantly positive or negative in 43 samples (71.7%). Of the 17 which differed, 14 bladder samples were positive and ear samples were negative while the converse occurred for three samples. Thus ear biopsy samples led to a significantly lower estimate of infection than bladder biopsy samples. This suggests that the use of ear biopsy samples in epidemiological studies of B. burgdorferi in Central European rodents is likely to lead to underestimates of the prevalence.
采用聚合酶链反应（PCR）比较了来自四种欧洲野生啮齿动物的膀胱和耳部活检样本中伯氏疏螺旋体狭义种的存在情况。在60对膀胱和耳部活检样本的比较中，43个样本（71.7%）的PCR结果一致为阳性或阴性。在17个结果不同的样本中，14个膀胱样本为阳性而耳部样本为阴性，另外3个样本则相反。因此，耳部活检样本得出的感染估计值显著低于膀胱活检样本。这表明，在中欧啮齿动物伯氏疏螺旋体的流行病学研究中，使用耳部活检样本可能会导致患病率的低估。
