Miyakawa T, Yagi T, Kagiyama A, Niki H
Department of Psychology, Faculty of Letters, University of Tokyo, Japan.
Brain Res Mol Brain Res. 1996 Apr;37(1-2):145-50. doi: 10.1016/0169-328x(95)00300-h.
Fyn-deficient mice produced by inserting the beta-galactosidase gene (lacZ) into the fyn gene locus were tested in a radial maze, an open field and an elevated plus-maze. In the radial maze, the homozygous Fyn-deficient (fynz/fynz) mice showed no impairment in spatial learning, although they showed a stronger avoidance tendency for those arms located closer to the experimenter during pretraining (adaptation). In the open-field test, the fynz/fynz mice defecated more frequently in the bright condition than did the +/fynz mice, and they were less active during the first 10-min test period than the +/fynz mice. In addition, the temporal pattern of locomotor activity for fynz/fynz mice was altered by changing the illumination while the pattern for +/fynz mice remained relatively unchanged. Scores reflecting the fear-response in the elevated plus-maze were higher in the fynz/fynz mice. These results indicate that Fyn deficiency increased fear-response, but did not impair spatial learning in the radial maze. It is suggested that we should take account of effects of emotional factors in the learning experiment using mutant mice.
通过将β-半乳糖苷酶基因（lacZ）插入Fyn基因位点而产生的Fyn缺陷小鼠，在放射状迷宫、旷场和高架十字迷宫中进行了测试。在放射状迷宫中，纯合Fyn缺陷（fynz/fynz）小鼠在空间学习方面没有受损，尽管在预训练（适应）期间，它们对靠近实验者的那些臂表现出更强的回避倾向。在旷场试验中，fynz/fynz小鼠在明亮条件下比+/fynz小鼠排便更频繁，并且在最初的10分钟测试期内比+/fynz小鼠活动更少。此外，改变光照时，fynz/fynz小鼠的运动活动时间模式发生了改变，而+/fynz小鼠的模式相对保持不变。反映高架十字迷宫中恐惧反应的分数在fynz/fynz小鼠中更高。这些结果表明，Fyn缺陷增加了恐惧反应，但没有损害放射状迷宫中的空间学习。建议在使用突变小鼠的学习实验中应考虑情绪因素的影响。
