Halpern H J, Peric M, Yu C, Barth E D, Chandramouli G V, Makinen M W, Rosen G M
Department of Radiation and Cellular Oncology, University of Chicago, Illinois 60637, USA.
Biophys J. 1996 Jul;71(1):403-9. doi: 10.1016/S0006-3495(96)79241-X.
A novel, very-low-frequency electron paramagnetic resonance (EPR) technique is used to image the distribution of several nitroxides with distinct pharmacologic compartment affinities in the abdomens of living mice. Image acquisition is sufficiently rapid to allow a time sequence of the distribution for each compound. The spectra and concentrations of these nitroxides are imaged with the use of spectral-spatial imaging to distinguish a single spatial dimension. Liver and bladder of the mouse anatomy are distinguished by this technique. After an intraperitoneal injection of the spin-label probes, a shift in the distribution of the compounds from the upper abdomen (primarily liver) to the lower abdomen (primarily bladder) is observed. The time dependence of the shift in regional distribution depends on the structural properties of the side chain attached to the spin label. These results indicate that this application of in vivo electron paramagnetic resonance imaging will provide a new method of magnetic resonance imaging for determination of pharmacodynamics in the body of an intact animal.
一种新型的甚低频电子顺磁共振（EPR）技术被用于对活小鼠腹部中具有不同药理学隔室亲和力的几种氮氧化物的分布进行成像。图像采集速度足够快，能够获取每种化合物分布的时间序列。利用光谱 - 空间成像对这些氮氧化物的光谱和浓度进行成像，以区分单一空间维度。通过这种技术可以区分小鼠解剖结构中的肝脏和膀胱。腹腔注射自旋标记探针后，观察到化合物分布从腹部上部（主要是肝脏）转移到腹部下部（主要是膀胱）。区域分布转移的时间依赖性取决于连接到自旋标记的侧链的结构特性。这些结果表明，体内电子顺磁共振成像的这种应用将为完整动物体内药效学的测定提供一种新的磁共振成像方法。
