Matsuda N, Ogura Y, Nishiwaki H, Miyamoto K, Matsubara T, Kiryu J, Honda Y
Department of Ophthalmology and Visual Sciences, Graduate School of Medicine, Kyoto University, Japan.
Invest Ophthalmol Vis Sci. 1996 Oct;37(11):2228-33.
To report a method to evaluate leukocyte dynamics in the choroidal circulation with indocyanine green (ICG) angiography.
Nonpigmented and pigmented rats were administered ICG solution intravenously. The fundus image was obtained with a scanning laser ophthalmoscope and recorded on magnetic tapes at a video rate (30 frames/second). The images were analyzed with a personal computer-based image analysis system.
On ICG angiography, hyperfluorescent dots were seen moving along the choroidal vessels and in the retinal vessels several minutes after injection. These fluorescent dots were thought to be circulating leukocytes stained with ICG. The micrographs of blood smears after ICG injection showed intense fluorescence of leukocytes. Computer-assisted image analysis allowed tracing of these fluorescent dots using a frame-by frame method.
Results of this study indicated that ICG can be used for vital staining of leukocytes and that it is possible to evaluate leukocyte movement in the choroidal circulation in vivo in rats.
报告一种用吲哚菁绿（ICG）血管造影术评估脉络膜循环中白细胞动力学的方法。
给无色素和有色素大鼠静脉注射ICG溶液。用扫描激光检眼镜获取眼底图像，并以视频速率（30帧/秒）记录在磁带上。用基于个人计算机的图像分析系统对图像进行分析。
在ICG血管造影中，注射后几分钟可见高荧光点沿脉络膜血管和视网膜血管移动。这些荧光点被认为是被ICG染色的循环白细胞。ICG注射后血涂片的显微照片显示白细胞有强烈荧光。计算机辅助图像分析允许使用逐帧方法追踪这些荧光点。
本研究结果表明ICG可用于白细胞的活体染色，并且有可能在大鼠体内评估脉络膜循环中白细胞的运动。
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