Rodionov D G, Ishiguro E E
Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada.
Antimicrob Agents Chemother. 1996 Apr;40(4):899-903. doi: 10.1128/AAC.40.4.899.
The role of protein synthesis in ampicillin-induced lysis of Escherichia coli was investigated. The inhibition of protein synthesis through amino acid deprivation resulted in the rapid development of ampicillin tolerance as a consequence of the stringent response, as previously reported. In contrast, inhibition of protein synthesis by use of ribosome inhibitors such as chloramphenicol did not readily confer ampicillin tolerance and, in fact, promoted the development of both stages of the ampicillin-induced lysis process, i.e., (i) an ampicillin-dependent stage which apparently involves the interaction of penicillin-binding proteins with ampicillin and (ii) an ampicillin-independent stage which may represent the events leading to the deregulation of peptidoglycan hydrolase activity. We propose that lysis was facilitated when protein synthesis was inhibited because the production of new penicillin-binding proteins to replace those which were ampicillin inhibited was prevented under these conditions.
研究了蛋白质合成在氨苄青霉素诱导大肠杆菌裂解中的作用。如先前报道，通过氨基酸剥夺抑制蛋白质合成会因严谨反应而迅速产生氨苄青霉素耐受性。相比之下，使用核糖体抑制剂如氯霉素抑制蛋白质合成并不能轻易赋予氨苄青霉素耐受性，事实上，反而促进了氨苄青霉素诱导裂解过程两个阶段的发展，即：（i）一个明显涉及青霉素结合蛋白与氨苄青霉素相互作用的氨苄青霉素依赖阶段，以及（ii）一个可能代表导致肽聚糖水解酶活性失调事件的氨苄青霉素非依赖阶段。我们认为，在这些条件下，当蛋白质合成受到抑制时裂解会更容易发生，因为新的青霉素结合蛋白的产生受到阻碍，无法替代那些被氨苄青霉素抑制的蛋白。
