吸附于人牙釉质的唾液乳过氧化物酶的酶活性。
Enzyme activity of salivary lactoperoxidase adsorbed to human enamel.
作者信息
Pruitt K M, Adamson M
出版信息
Infect Immun. 1977 Jul;17(1):112-6. doi: 10.1128/iai.17.1.112-116.1977.
Abstract
Human saliva was incubated with human enamel powder, and the lactoperoxidase activity of the saliva was measured before and after incubation. Liquidphase lactoperoxidase activity was reduced in direct proportion to the weight of enamel powder added. Lactoperoxidase molecules were adsorbed to the enamel surface in an enzymatically active conformation, and this enamel-bound lactoperoxidase activity was also measured. The adsorption of lactoperoxidase was irreversible and produced at least a 40% increase in the concentration of lactoperoxidase in the enamel surface phase as compared with its concentration in the liquid phase. Enamel-bound lactoperoxidase, as well as the free enzyme, was capable of inactivating the key glycolytic enzyme hexokinase. The implications of the adsorption phenomenon for bacterial colonization are discussed.
摘要
将人类唾液与人类牙釉质粉末一起孵育，并在孵育前后测量唾液中的乳过氧化物酶活性。液相乳过氧化物酶活性与添加的牙釉质粉末重量成反比降低。乳过氧化物酶分子以具有酶活性的构象吸附到牙釉质表面，并且还测量了这种与牙釉质结合的乳过氧化物酶活性。乳过氧化物酶的吸附是不可逆的，并且与液相中其浓度相比，牙釉质表面相中乳过氧化物酶的浓度至少增加了40%。与牙釉质结合的乳过氧化物酶以及游离酶都能够使关键的糖酵解酶己糖激酶失活。讨论了吸附现象对细菌定植的影响。
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