使用万古霉素筛选琼脂检测肠球菌的接种方法比较
Comparison of inoculation methods for testing enterococci by using vancomycin screening agar.
作者信息
Jorgensen J H, McElmeel M L, Trippy C W
机构信息
Department of Pathology, University of Texas, Health Science Center, San Antonto 78284-7750, USA.
出版信息
J Clin Microbiol. 1996 Nov;34(11):2841-2. doi: 10.1128/jcm.34.11.2841-2842.1996.
Abstract
One hundred four recent clinical isolates of Enterococcus species were screened for vancomycin resistance by using inocula of 10(5) or 10(6) CFU dispensed by pipet and by use of a cotton swab dipped in a 0.5 McFarland standard organism suspension applied to the surface of brain heart infusion agar containing 6 micrograms of vancomycin per ml. The three inoculation methods were equivalent in the detection of nonsusceptible isolates. The use of swab inoculation was convenient and less costly than the use of micropipets.
摘要
通过使用移液器接种10⁵或10⁶CFU的接种物，以及使用蘸有0.5麦氏标准菌悬液的棉拭子涂于每毫升含6微克万古霉素的脑心浸液琼脂表面，对104株近期分离的肠球菌临床菌株进行万古霉素耐药性筛查。这三种接种方法在检测非敏感菌株方面效果相当。使用拭子接种比使用微量移液器更方便且成本更低。
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本文引用的文献
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