使用阿拉玛微量肉汤稀释法检测肠球菌对万古霉素的耐药性。
Detection of vancomycin resistance in enterococci by the Alamar MIC system.
作者信息
Zabransky R J, Dinuzzo A R, Woods G L
机构信息
Department of Pathology, University of Texas Medical Branch, Galveston 77555-0743, USA.
出版信息
J Clin Microbiol. 1995 Apr;33(4):791-3. doi: 10.1128/jcm.33.4.791-793.1995.
Abstract
The ability of the Alamar microdilution MIC system to detect vancomycin resistance in enterococci was evaluated by comparing the results with an agar dilution screen method. Of 100 strains tested, 41 were resistant and 47 were susceptible by both tests. Five strains were intermediate and one was resistant by the Alamar MIC system but susceptible by the agar screen. Three strains each were susceptible or intermediate by the Alamar MIC system but resistant by the agar screen. The predictive values for the Alamar MIC system were 94% (susceptible) and 88% (combined intermediate and resistant). The Alamar MIC system does not appear to have sufficient accuracy for the detection or confirmation of vancomycin resistance in enterococci.
摘要
通过将结果与琼脂稀释筛选法进行比较，评估了阿拉玛微量稀释法MIC系统检测肠球菌对万古霉素耐药性的能力。在测试的100株菌株中，两种测试方法均显示41株耐药，47株敏感。5株为中介型，1株阿拉玛MIC系统检测为耐药，但琼脂筛选法检测为敏感。阿拉玛MIC系统检测为敏感的预测值为94%，检测为中介型和耐药的预测值为88%。阿拉玛MIC系统在检测或确认肠球菌对万古霉素的耐药性方面似乎没有足够的准确性。
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