斑马鱼嗅觉上皮中气味受体的嵌套表达域。
Nested expression domains for odorant receptors in zebrafish olfactory epithelium.
作者信息
Weth F, Nadler W, Korsching S
机构信息
Max-Planck-Institut für Entwicklungsbiologie, Abteilung Physikalische Biologie, Tübingen, Germany.
出版信息
Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):13321-6. doi: 10.1073/pnas.93.23.13321.
Abstract
The mapping of high-dimensional olfactory stimuli onto the two-dimensional surface of the nasal sensory epithelium constitutes the first step in the neuronal encoding of olfactory input. We have used zebrafish as a model system to analyze the spatial distribution of odorant receptor molecules in the olfactory epithelium by quantitative in situ hybridization. To this end, we have cloned 10 very divergent zebrafish odorant receptor molecules by PCR. Individual genes are expressed in sparse olfactory receptor neurons. Analysis of the position of labeled cells in a simplified coordinate system revealed three concentric, albeit overlapping, expression domains for the four odorant receptors analyzed in detail. Such regionalized expression should result in a corresponding segregation of functional response properties. This might represent the first step of spatial encoding of olfactory input or be essential for the development of the olfactory system.
摘要
将高维嗅觉刺激映射到鼻感觉上皮的二维表面是嗅觉输入神经元编码的第一步。我们以斑马鱼为模型系统，通过定量原位杂交分析嗅觉上皮中气味受体分子的空间分布。为此，我们通过聚合酶链反应（PCR）克隆了10个差异很大的斑马鱼气味受体分子。单个基因在稀疏的嗅觉受体神经元中表达。在一个简化的坐标系中分析标记细胞的位置，发现详细分析的四种气味受体有三个同心但重叠的表达域。这种区域化表达应导致功能反应特性的相应分离。这可能代表嗅觉输入空间编码的第一步，或者对嗅觉系统的发育至关重要。
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